Q73CE0 (GLPK_BACC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BCE_1125

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Glycerol kinase HAMAP MF_00186		PRO_1000020699
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73CE0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6ACBF18E0ABD8ACB	FASTA	510	56,912
	10 20 30 40 50 60 MKKYILSLDQ GTTSSRAILX NKKGEIVHSA QKEFTQHFPK PGWVEHNAQE IWGSILAVIA 70 80 90 100 110 120 TCLSEADVKP EQIAGIGITN QRETAVVWDK TTGKPIYNAI VWQSRQTAEI CDELKEKGYG 130 140 150 160 170 180 EMVREKTGLL IDAYFSGTKV KWILDNVEGA REKAENGDLL FGTIDTWLVW KLSGGKAHVT 190 200 210 220 230 240 DYSNASRTLM FNIHDLQWDD ELLDMLTVPK SMLPEVRPSS EVYGETIDYH FFGQNVPIAG 250 260 270 280 290 300 VAGDQQAALF GQACFGEGMA KNTYGTGCFM LMNTGEKAVA SEHGLLTTIA WGIDGKVNYA 310 320 330 340 350 360 LEGSIFVAGS AIQWLRDGMR MFKDASESEV YANRVESTDG VYVVPAFVGL GTPYWDSEVR 370 380 390 400 410 420 GAMFGVTRGT TKEHFIRATL ESLAYQXKDV LCAMEADSGI ELKTLRVDGG AVKNNFLMKF 430 440 450 460 470 480 QSDILDVPVE RPVINETTAL GAAYLAGLAV GYWKNQDEIK EQWHMDKRLN QQWKRKQAKS 490 500 510 YMLDGKKQLK QQKLSNNHKQ CYNGDKLIIR

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS40056.1.
RefSeq	NP_977448.1. NC_003909.8.
3D structure databases
HSSP	HSSP built from PDB template 1XUP based on UniProtKB O34153.
ProteinModelPortal	Q73CE0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q73CE0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000029401; EBBACP00000028751; EBBACG00000029392.
GeneID	2750781.
GenomeReviews	Gene locus BCE_1125 in contig AE017194_GR.
KEGG	bca:BCE_1125.
NMPDR	fig|222523.1.peg.1120.
PATRIC	18851122. VBIBacCer118379_1066.
TIGR	BCE_1125.
Phylogenomic databases
eggNOG	COG0554.
GeneTree	EBGT00050000001374.
HOGENOM	HBG511469.
OMA	ALYGQLC.
ProtClustDB	PRK00047.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BACC1

Accession

Primary (citable) accession number: Q73CE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program