Reviewed, UniProtKB/Swiss-Prot Q73BU4 (PRPE_BACC1)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
    EC=3.6.1.17
Alternative name(s):
    Ap4A hydrolase
    Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
      Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	BCE_1324

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: HAMAP nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_0000297696

Sequences

	Sequence		Length	Mass (Da)
	Q73BU4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6D26BA9B4189D21F	FASTA	246	28,279
	10 20 30 40 50 60 MKYDIIGDIH GCFQEFQDLT KKLGYNWNSD LPIHPDQRKL AFVGDITDRG PHSLRMIEIV 70 80 90 100 110 120 WELVINKKVA YYAPGNHCNK LYRFFLGRNV TIAHGLETTV AEYEALPSHQ QNMIKEKFIT 130 140 150 160 170 180 LYEQSPLYHV LDEKRLIVCH AGIRQDYIGR QDKKVQTFVL YGDITGEKHA DGSPVRRDWA 190 200 210 220 230 240 KEYKGTAWVV YGHTPVKEPR FVNHTVNIDT GAVFGGKLTG LRYPEMETVS VPSSLPFVPE KFRPIS

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS40253.1.
RefSeq	NP_977645.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q73BU4.
Genome annotation databases
GeneID	2748994.
GenomeReviews	Gene locus BCE_1324 in contig AE017194_GR.
KEGG	bca:BCE_1324.
NMPDR	fig|222523.1.peg.1317.
TIGR	BCE_1324.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HBG724457.
OMA	HCNKLYR.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR004843. M-pesterase. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
PANTHER	PTHR11668. T_phtase_apaH. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_BACC1

Accession

Primary (citable) accession number: Q73BU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)