Reviewed, UniProtKB/Swiss-Prot Q73A47 (ILVC2_BACC1)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase 2
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase 2
    Alpha-keto-beta-hydroxylacil reductoisomerase 2

Gene names

Name:	ilvC2
Ordered Locus Names:	BCE_1936

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Ketol-acid reductoisomerase 2		PRO_0000151273
Sites
	Active site	106	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q73A47-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E3424127E43BDEE4	FASTA	335	36,898
	10 20 30 40 50 60 MKTYYEKDAN VELLKGKTVA VIGYGSQGHA QAQNLRDSGV EVVVGVRPGK SFEVAKTDGF 70 80 90 100 110 120 EVMSVSEAVR TAQVVQMLLP DEQQAHVYKA GVEENLREGQ MLLFSHGFNI HFGQINPPSY 130 140 150 160 170 180 VDVAMVAPKS PGHLVRRVFQ EGNGVPALVA VHQDATGTAL HVALAYAKGV GCTRAGVIET 190 200 210 220 230 240 TFQEETETDL FGEQTVLCGG VTALVKAGFE TLTEGGYRPE IAYFECLHEL KLIVDLMYEG 250 260 270 280 290 300 GLTNMRHSIS DTAEFGDYVT GSRIVTDETK KEMKRVLTEI QQGEFAKKWI LENQAGRPTY 310 320 330 NAMKKAEQNH QLEKVGAELR EMMSWIDAPK ELVKK

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017194 Genomic DNA. Translation: AAS40860.1.
RefSeq	NP_978252.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2747189.
GenomeReviews	Gene locus BCE_1936 in contig AE017194_GR.
KEGG	bca:BCE_1936.
NMPDR	fig|222523.1.peg.1924.
TIGR	BCE_1936.
Phylogenomic databases
HOGENOM	Q73A47.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC2_BACC1

Accession

Primary (citable) accession number: Q73A47

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)