Q739R5 (NADE_BACC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BCE_2075

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077533
Regions
	Nucleotide binding	45 – 52	8	ATP By similarity
Sites
	Active site	47	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q739R5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 62FE2BD7DA4EBFB5	FASTA	272	30,059
	10 20 30 40 50 60 MTLQEQIMKA LHVQPVIDPK AEIRKRVDFL KDYVKKTGAK GFVLGISGGQ DSTLAGRLAQ 70 80 90 100 110 120 LAVEEIRNEG GNATFIAVRL PYKVQKDEDD AQLALQFIQA DQSVAFDIAS TVDAFSNQYE 130 140 150 160 170 180 NLLGESLTDF NKGNVKARIR MVTQYAIGGQ NSLLVIGTDH AAEAVTGFFT KFGDGGADLL 190 200 210 220 230 240 PLTGLTKRQG RALLQELGAD ERLYLKMPTA DLLDEKPGQA DETELGITYD QLDDYLEGKA 250 260 270 VPTDVAEKIE KRYTVSEHKR QVPASMFDDW WK

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS40996.1.
RefSeq	NP_978388.1. NC_003909.8.
3D structure databases
HSSP	HSSP built from PDB template 1IH8 based on UniProtKB P08164.
ProteinModelPortal	Q739R5.
SMR	Q739R5. Positions 1-272.
ModBase	Search...
Protein-protein interaction databases
STRING	Q739R5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000025057; EBBACP00000024407; EBBACG00000025048.
GeneID	2747931.
GenomeReviews	Gene locus BCE_2075 in contig AE017194_GR.
KEGG	bca:BCE_2075.
NMPDR	fig|222523.1.peg.2060.
PATRIC	18852961. VBIBacCer118379_1983.
TIGR	BCE_2075.
Phylogenomic databases
eggNOG	COG0171.
GeneTree	EBGT00050000001308.
HOGENOM	HBG351567.
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	BCER405917:BCE_2075-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BACC1

Accession

Primary (citable) accession number: Q739R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program