Q739N4 (NAMA_BACC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Alternative name(s):
Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	BCE_2106

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614
Cofactor	FMN By similarity. HAMAP MF_01614
Subunit structure	Homotetramer By similarity. HAMAP MF_01614
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	NADPH dehydrogenase HAMAP MF_01614		PRO_0000216113
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q739N4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 70331CB291BB1E16	FASTA	345	38,536
	10 20 30 40 50 60 MNYKLFSPYT IKDVTLKNRI VMSPMCMYSS ENGDGQVTNF HLIHYGTRAA GQVGLVMIEA 70 80 90 100 110 120 TAVLPEGRIS NKDLGIWDDS LIEGLHKTTT FIHDNGAKAA IQLAHAGRKA ELETDALAPS 130 140 150 160 170 180 AIPFNETMKM PIEMSKHQIK DTVLAFQQAA VRSKQAGFDV IEIHGAHGYL INEFLSPLTN 190 200 210 220 230 240 KRTDEYGGSP ENRYRFLREI IDSINEVWNG PLFVRISAND YHPDGLTVQD YVQYTKWMKE 250 260 270 280 290 300 QGVDLIDCSS GAVVPARIDV YPGYQVQYAK HIKEHANIAT GAVGLITTGA QAEQILNNNE 310 320 330 340 ADLIFIGREL LRNPYFPRIA ANELGFELEE PYQYERAPGK ISTNK

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS41027.1.
RefSeq	NP_978419.1. NC_003909.8.
3D structure databases
ProteinModelPortal	Q739N4.
SMR	Q739N4. Positions 4-337.
ModBase	Search...
Protein-protein interaction databases
STRING	Q739N4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000029744; EBBACP00000029094; EBBACG00000029735.
GeneID	2752685.
GenomeReviews	Gene locus BCE_2106 in contig AE017194_GR.
KEGG	bca:BCE_2106.
NMPDR	fig|222523.1.peg.2091.
PATRIC	18853020. VBIBacCer118379_2012.
TIGR	BCE_2106.
Phylogenomic databases
eggNOG	COG1902.
GeneTree	EBGT00050000002219.
HOGENOM	HBG583461.
OMA	WVEDGWN.
ProtClustDB	PRK13523.
Enzyme and pathway databases
BioCyc	BCER405917:BCE_2106-MONOMER.
Family and domain databases
HAMAP	MF_01614. NamA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00356.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_BACC1

Accession

Primary (citable) accession number: Q739N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program