Reviewed, UniProtKB/Swiss-Prot Q739J0 (3HAO_BACC1)

Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Ordered Locus Names:

BCE_2151

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity.
Catalytic activity	3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde. HAMAP MF_00825
Cofactor	Binds 2 Fe2+ ions per subunit By similarity.
Sequence similarities	Belongs to the 3-HAO family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: HAMAP ferrous iron binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	3-hydroxyanthranilate 3,4-dioxygenase HAMAP MF_00825		PRO_0000245473
Sites
	Metal binding	51	1	Iron 1; catalytic By similarity
	Metal binding	57	1	Iron 1; catalytic By similarity
	Metal binding	96	1	Iron 1; catalytic By similarity
	Metal binding	125	1	Iron 2 By similarity
	Metal binding	128	1	Iron 2 By similarity
	Metal binding	162	1	Iron 2 By similarity
	Metal binding	165	1	Iron 2 By similarity
	Binding site	47	1	Dioxygen By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q739J0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1A4A10AA8AC192AC	FASTA	179	21,084
	10 20 30 40 50 60 MSKTLQSFNL LKWIDENKEL LKPPVNNKVI WQDSEFIAMI LGGPNRRRDF HVDPSDEFFY 70 80 90 100 110 120 QIKGECYVEC ITEEGKREVV TVKEGDVFML PAMVPHSPHR VANTYGLVIE RKRNQGELED 130 140 150 160 170 FVWFCDECNH EMHRVRVQLS DIEKQVKEAI HSFNSNKEIR ACKNCGHIMP EEVEEWKCE

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017194 Genomic DNA. Translation: AAS41072.1.
RefSeq	NP_978464.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2748280.
GenomeReviews	Gene locus BCE_2151 in contig AE017194_GR.
KEGG	bca:BCE_2151.
NMPDR	fig|222523.1.peg.2136.
TIGR	BCE_2151.
Phylogenomic databases
HOGENOM	Q739J0.
OMA	Q739J0. RHSPQRP.
Family and domain databases
HAMAP	MF_00825. [Tree]
InterPro	IPR010329. 3hydroanth_dOase. [Graphical view]
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3HAO_BACC1

Accession

Primary (citable) accession number: Q739J0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)