Reviewed, UniProtKB/Swiss-Prot Q739A1 (SYI2_BACC1)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase 2
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase 2
      Short name=IleRS 2

Gene names

Name:	ileS2
Ordered Locus Names:	BCE_2241

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02003
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003
Cofactor	Zinc By similarity. HAMAP MF_02003
Subunit structure	Monomer By similarity. HAMAP MF_02003
Subcellular location	Cytoplasm By similarity HAMAP MF_02003.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02003
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1033	1033	Isoleucyl-tRNA synthetase 2 HAMAP MF_02003		PRO_0000098516
Regions
	Motif	47 – 57	11	"HIGH" region HAMAP MF_02003
	Motif	590 – 594	5	"KMSKS" region HAMAP MF_02003
Sites
	Binding site	593	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q739A1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C75BD19783337160	FASTA	1,033	118,420
	10 20 30 40 50 60 MKKVDVKESA VGRETRIRKQ WNEQSIFEQS IQNREGAQSF VFYEGPPTAN GLPHVGHALG 70 80 90 100 110 120 RTIKDVVARY KTMAGYKVLR KAGWDTHGLP VELGVEKQLG ISGKHEIEEY GIEPFIKKCK 130 140 150 160 170 180 ESVFTYEKQW REFTESIGYW VDMDDPYVTL ENPYIESVWH ILGTIHEKGL LYKGHRVSPY 190 200 210 220 230 240 CPSCQTSLSS HEVAQGYKTV KDLSATVKFK VKDSENEYFL GWTTTPWTLP ANVALAVHPN 250 260 270 280 290 300 MEYVKAKQEG YVYIVAKERV QDVLKEDYEV LSVHKGEELV NTSYTAPFPM KEVTNGYHVI 310 320 330 340 350 360 AADFVTADSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEAVP FLKGKFVKDS 370 380 390 400 410 420 DVDIVRYLAK EGLLYHKEKY EHSYPHCWRC DSPLLYYAGE SWLIRTTAIK DTFLQNNDTV 430 440 450 460 470 480 TWYPDHMKHG RFGKFLENMV DWNISRNRYW GTPLNVWECE SCDHQFAPKS IADLRKHSTK 490 500 510 520 530 540 ETPEDLELHK PYVDEVQVSC EKCGGAMNRT PEVIDVWFDS GSMPFAQYHY PFENKELFED 550 560 570 580 590 600 QFPADVIAEG IDQTRGWFYS LLAVSALYTG KVPYKRVLSL GHVLDEEGQK MSKSKGNALD 610 620 630 640 650 660 PVDLVNQFGA DALRWALLVD SAPWNAKRFS ERTVLEAKSK FVDTLVNVYS FYVLYANLDE 670 680 690 700 710 720 YNPDETYDVK RTKLDEWVLS RLHSTTKKVR TALDDYQFTN AAREIATLVD EVSNWYVRRS 730 740 750 760 770 780 RNRFWESGMN AEKAAAYETL HEVLVTISKL IAPFTPFVAE DIHLNLTGSS VHLEDYPVVN 790 800 810 820 830 840 ESLLQPKLEA EMDAVLQVVE LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM 850 860 870 880 890 900 DELNVKAFHV ELDETKYTSY QLKLNFKTAG PKFGKNVNAV NDWLKQLSQD EVQNFVTTER 910 920 930 940 950 960 AVYEAASGEE VVVTTEDVLV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ EGVAREFIRA 970 980 990 1000 1010 1020 VQEYRKQLNL PVNLRVDVIL DTEEELQQTL TNHKQLLEEN LLVKQFTFGH LTNEDDELSL 1030 GETKVRIKLS ATK

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS41161.1.
RefSeq	NP_978553.1.
3D structure databases
SMR	Q739A1. Positions 10-810.
ModBase	Search...
Protein-protein interaction databases
STRING	Q739A1.
Genome annotation databases
GeneID	2749305.
GenomeReviews	Gene locus BCE_2241 in contig AE017194_GR.
KEGG	bca:BCE_2241.
NMPDR	fig|222523.1.peg.2225.
TIGR	BCE_2241.
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HBG577712.
OMA	KENGAFT.
Family and domain databases
HAMAP	MF_02003. Ile_tRNA_synth_type2. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI2_BACC1

Accession

Primary (citable) accession number: Q739A1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)