Q732N7 (Q732N7_BACC1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL AAS42780.1
EC=5.99.1.2 EMBL AAS42780.1

Gene names

Name:	topA EMBL AAS42780.1
Ordered Locus Names:	BCE_3875

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP] EMBL AAS42780.1

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	692 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q732N7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4C09B6F47D8ADBF7	FASTA	692	79,105
	10 20 30 40 50 60 MSDYLVIVES PSKAKTIEKY LGKKYKVVAS MGHVRDLPKS QMGIEVKNNF TPKYITIRGK 70 80 90 100 110 120 GPVLKDLKSA AKKAKKVYLA ADPDREGEAI AWHLANTLNV DVESDCRVVF NEITKDAIKE 130 140 150 160 170 180 SFKHPRAINM NLVDAQQARR ILDRLVGYNI SPLLWKKVKK GLSAGRVQSV AVRLIIERER 190 200 210 220 230 240 EIQSFEPEEF WTIKTEFVKG KDTFEASFYG VDGEKVQLTN ETQVNEIIER LKDNAFSVEN 250 260 270 280 290 300 VTRKERKRNP ALPFTTSSLQ QEAARKLNMR AKKTMMLAQQ LYEGIDLGKQ GTVGLITYMR 310 320 330 340 350 360 TDSTRISETA QTEARTYITE AFGTEYIGTE KKKETKKSNA QDAHEAIRPT SVMRKPEELK 370 380 390 400 410 420 SFLSRDQLRL YKLIWERFVA SQMASAIMDT VTARLINNNV QFRASGSVVK FPGFMKVYVE 430 440 450 460 470 480 SKDDGAEEKD KMLPPLEVGE TVFSKDLEPK QHFTQPPPRY TEARLVRTLE ELGIGRPSTY 490 500 510 520 530 540 VPTLETIQKR GYVGLDNKRF VPTELGEIVI ELILEFFPEI INIEFTANME QSLDEVEEGN 550 560 570 580 590 600 ANWVKIVDDF YVGFEPRLEK AEKEMREVEI KDEPAGEDCE LCNHPMVFKM GKYGKFMACS 610 620 630 640 650 660 NFPDCRNTKP IVKEIGVTCP KCDKGQIIER RSNKKKRLFY GCGTYPECDF VSWDKPIGRK 670 680 690 CPKCEGMLVE KKLKKGVQVQ CISCDYEEEQ QM

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS42780.1.
RefSeq	NP_980172.1. NC_003909.8.
3D structure databases
ProteinModelPortal	Q732N7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q732N7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000027810; EBBACP00000027160; EBBACG00000027801.
GeneID	2747827.
GenomeReviews	Gene locus BCE_3875 in contig AE017194_GR.
KEGG	bca:BCE_3875.
NMPDR	fig|222523.1.peg.3844.
PATRIC	18856406. VBIBacCer118379_3702.
TIGR	BCE_3875.
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00070000032133.
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q732N7_BACC1

Accession

Primary (citable) accession number: Q732N7

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)