Reviewed, UniProtKB/Swiss-Prot Q732I7 (PYRE_BACC1)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BCE_3927

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110667
Regions
	Region	120 – 128	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	94	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	98	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	100	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	124	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q732I7-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: FBFCBB786C852A4B	FASTA	210	22,702
	10 20 30 40 50 60 MKKEIASHLL EIGAVFLQPN DPFTWSSGMK SPIYCDNRLT LSYPKVRQAI AAGLEELIKE 70 80 90 100 110 120 HFPTVEVIAG TATAGIAHAA WVSDRMDLPM CYVRSKAKGH GKGNQIEGKA EKGQKVVVVE 130 140 150 160 170 180 DLISTGGSAI TCVEALREAG CEVLGIVSIF TYELEAGKEK LEAANVASYS LSDYSALTEV 190 200 210 AAEKGMIGQA ETKKLQEWRK DPANEAWITA

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS42830.1.
RefSeq	NP_980222.1.
3D structure databases
SMR	Q732I7. Positions 1-204.
ModBase	Search...
Protein-protein interaction databases
STRING	Q732I7.
Genome annotation databases
GeneID	2752532.
GenomeReviews	Gene locus BCE_3927 in contig AE017194_GR.
KEGG	bca:BCE_3927.
NMPDR	fig|222523.1.peg.3894.
TIGR	BCE_3927.
Phylogenomic databases
eggNOG	COG0461.
HOGENOM	HBG404341.
OMA	PMCYVRS.
Family and domain databases
HAMAP	MF_01208_B. PyrE_B. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BACC1

Accession

Primary (citable) accession number: Q732I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)