Reviewed, UniProtKB/Swiss-Prot Q732H6 (LSPA_BACC1)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BCE_3938

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity.
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Lipoprotein signal peptidase HAMAP MF_00161		PRO_0000289350
Regions
	Transmembrane	55 – 75	21	Potential
	Transmembrane	85 – 105	21	Potential
	Transmembrane	124 – 144	21	Potential
Sites
	Active site	102	1	By similarity
	Active site	129	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q732H6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C7AE046FC2CFCA4C	FASTA	152	17,426
	10 20 30 40 50 60 MIYYVIALFV IAIDQISKWL IVKNMELGTS IPIIDNVLYI TSHRNRGAAW GILENKMWFF 70 80 90 100 110 120 YIITVVFVAF IVFYMKKYAK TDKLLGISLG LILGGAIGNF IDRVFRQEVV DFIHVYIFSY 130 140 150 NYPVFNIADS ALCIGVVLII IQTLLEGKKT KE

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017194 Genomic DNA. Translation: AAS42841.1.
RefSeq	NP_980233.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q732H6.
Protein family/group databases
MEROPS	A08.001.
Genome annotation databases
GeneID	2751837.
GenomeReviews	Gene locus BCE_3938 in contig AE017194_GR.
KEGG	bca:BCE_3938.
NMPDR	fig|222523.1.peg.3905.
TIGR	BCE_3938.
Phylogenomic databases
HOGENOM	Q732H6.
OMA	ADSSGWQ.
Family and domain databases
HAMAP	MF_00161. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
ProDom	PD004304. Peptidase_A8. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BACC1

Accession

Primary (citable) accession number: Q732H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)