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Q732F6 (MURD_BACC1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase

EC=6.3.2.9
Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Gene names
Name:murD
Ordered Locus Names:BCE_3958
OrganismBacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]
Taxonomic identifier222523 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639

Subcellular location

Cytoplasm By similarity HAMAP MF_00639.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639
PRO_0000108960

Regions

Nucleotide binding119 – 1257ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q732F6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: F708DA8DF90BF415

FASTA45048,812
        10         20         30         40         50         60 
MKTVTEFQNK NILVLGIAKS GYAAATLLQK LGANVIVNDG KPLAENVLAA ELQAKGMDVV 

        70         80         90        100        110        120 
CGGHPLELLE RNISLVVKNP GIPYSNPILV AAKEKQIPIV TEVELAYRIS EAPFVGITGS 

       130        140        150        160        170        180 
NGKTTTTMLT FEMLKEGQKH PVIAGNIGTV ACEVAQDAKE NEVVVTELSS FQLMGVELFQ 

       190        200        210        220        230        240 
PKIAAFLNLF EAHLDYHGTK KEYGLAKANI FKNXTENDYS VINADDADVM ALSAYSKGQK 

       250        260        270        280        290        300 
VLFSTTKEIE DGACIKDNAL YFKGEKVVEV GDIVLPGQHN LENILAAMSI AKLLGVSNEA 

       310        320        330        340        350        360 
ITAVLKRFTG VKHRLEYVTT INNRKFYNDS KATNMLATEK ALSAFTQPTV LLAGGLDRGN 

       370        380        390        400        410        420 
EFDDLIPYFK NVKAIVTFGQ TAPKLVRAAE KAGLDTIESV DTLDEAVVKA YAHSTDGDVI 

       430        440        450 
LLSPACASWD QFKTFEERGD IFIQAVHKLI 

« Hide

References

[1]"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1."
Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D.
Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 10987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017194 Genomic DNA. Translation: AAS42861.1.
RefSeqNP_980253.1. NC_003909.8.

3D structure databases

ProteinModelPortalQ732F6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ732F6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000027120; EBBACP00000026470; EBBACG00000027111.
GeneID2750493.
GenomeReviewsGene locus BCE_3958 in contig AE017194_GR.
KEGGbca:BCE_3958.
NMPDRfig|222523.1.peg.3925.
PATRIC18856570. VBIBacCer118379_3784.
TIGRBCE_3958.

Phylogenomic databases

eggNOGCOG0771.
GeneTreeEBGT00070000032130.
HOGENOMHBG750024.
OMAACASWDM.
ProtClustDBPRK02472.

Enzyme and pathway databases

BioCycBCER405917:BCE_3958-MONOMER.

Family and domain databases

HAMAPMF_00639. MurD.
[Tree]
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
Gene3DG3DSA:3.90.190.20. Mur_ligase_C. 1 hit.
G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK01925.
PANTHERPTHR23135:SF2. PTHR23135:SF2. 1 hit.
PfamPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMsTIGR01087. MurD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURD_BACC1
AccessionPrimary (citable) accession number: Q732F6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families