Q732F6 (MURD_BACC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	BCE_3958

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108960
Regions
	Nucleotide binding	119 – 125	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q732F6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: F708DA8DF90BF415	FASTA	450	48,812
	10 20 30 40 50 60 MKTVTEFQNK NILVLGIAKS GYAAATLLQK LGANVIVNDG KPLAENVLAA ELQAKGMDVV 70 80 90 100 110 120 CGGHPLELLE RNISLVVKNP GIPYSNPILV AAKEKQIPIV TEVELAYRIS EAPFVGITGS 130 140 150 160 170 180 NGKTTTTMLT FEMLKEGQKH PVIAGNIGTV ACEVAQDAKE NEVVVTELSS FQLMGVELFQ 190 200 210 220 230 240 PKIAAFLNLF EAHLDYHGTK KEYGLAKANI FKNXTENDYS VINADDADVM ALSAYSKGQK 250 260 270 280 290 300 VLFSTTKEIE DGACIKDNAL YFKGEKVVEV GDIVLPGQHN LENILAAMSI AKLLGVSNEA 310 320 330 340 350 360 ITAVLKRFTG VKHRLEYVTT INNRKFYNDS KATNMLATEK ALSAFTQPTV LLAGGLDRGN 370 380 390 400 410 420 EFDDLIPYFK NVKAIVTFGQ TAPKLVRAAE KAGLDTIESV DTLDEAVVKA YAHSTDGDVI 430 440 450 LLSPACASWD QFKTFEERGD IFIQAVHKLI

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS42861.1.
RefSeq	NP_980253.1. NC_003909.8.
3D structure databases
ProteinModelPortal	Q732F6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q732F6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000027120; EBBACP00000026470; EBBACG00000027111.
GeneID	2750493.
GenomeReviews	Gene locus BCE_3958 in contig AE017194_GR.
KEGG	bca:BCE_3958.
NMPDR	fig|222523.1.peg.3925.
PATRIC	18856570. VBIBacCer118379_3784.
TIGR	BCE_3958.
Phylogenomic databases
eggNOG	COG0771.
GeneTree	EBGT00070000032130.
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	PRK02472.
Enzyme and pathway databases
BioCyc	BCER405917:BCE_3958-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BACC1

Accession

Primary (citable) accession number: Q732F6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program