Q731W2 (METE_BACC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BCE_4053

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	762 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 762	762	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098610
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q731W2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6E7C9A024EB8EBBB	FASTA	762	87,325
	10 20 30 40 50 60 MAIQTSNLGY PRIGLQREWK KTLEAFWSNK IDEEQFLTTM KEIRLQHVKA QQEKGIELIP 70 80 90 100 110 120 IGDFTYYDHV LDTAYMLGFI PSRFSEFTSY LDVYFAMARG SKDHVASEMT KWFNTNYHYI 130 140 150 160 170 180 VPEYEEGLQI SLKDNRPLRL YEEAKQELGI DGKPVILGPY TFLKLAKGYT QEQFATILKQ 190 200 210 220 230 240 LVAPYVQLLS ELHAAGAQII QVDEPIFASL TKEEVQQAKE IYEAIRKEVP NANLLLQTYF 250 260 270 280 290 300 DSVEENYEEI ITFPVSSIGL DFVHGKEGNL HAISKYGFPA DKTLAVGCID GRNIWRADLD 310 320 330 340 350 360 EVLTLFTTLQ KQVQTKDFIV QPSCSLLHTP IDKTEETHLS TELFDALAFA NQKLEELVLI 370 380 390 400 410 420 HSALTQGTES IHNELETYRN VHHTIRSSAA RNREDVKAAR TALKEEDFSR PLPFEKRYEL 430 440 450 460 470 480 QQVALKLPLL PTTTIGSFPQ TTEVRQTRKE WRNGVISNEQ YEQFIEKETE KWIRYQEEIG 490 500 510 520 530 540 LDVLVHGEFE RTDMVEYFGE RLAGFSFTKN GWVQSYGSRC VKPPVIYGDV AFINGMTIKE 550 560 570 580 590 600 TVYAQSLTEK VVKGMLTGPV TILNWSFVRN DIPRKEVSYQ IALALRHEIE LLESSGIRVI 610 620 630 640 650 660 QVDEPALREG MPLKEKDWDA YITWAVQSFL LATSSVANET QIHTHMCYSN FEDIVDAIRA 670 680 690 700 710 720 LDADVISIET SRSHGEFIDT LKHTTYEKGI GLGVYDIHSP RVPSKDEMYK IVEQSLKVCD 730 740 750 760 PKYFWINPDC GLKTRRTEEV IPALEHMVQA AKDARSLLKT NA

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS42955.1.
RefSeq	NP_980347.1. NC_003909.8.
3D structure databases
ProteinModelPortal	Q731W2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q731W2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000027379; EBBACP00000026729; EBBACG00000027370.
GeneID	2748766.
GenomeReviews	Gene locus BCE_4053 in contig AE017194_GR.
KEGG	bca:BCE_4053.
NMPDR	fig|222523.1.peg.4019.
PATRIC	18856757. VBIBacCer118379_3877.
TIGR	BCE_4053.
Phylogenomic databases
eggNOG	COG0620.
GeneTree	EBGT00050000001686.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BCER405917:BCE_4053-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BACC1

Accession

Primary (citable) accession number: Q731W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program