Q731P4 (NAGB_BACC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucosamine-6-phosphate deaminase
EC=3.5.99.6

Alternative name(s):
GlcN6P deaminase
Short name=GNPDA
Glucosamine-6-phosphate isomerase

Gene names

Name:	nagB
Ordered Locus Names:	BCE_4121

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion By similarity. HAMAP-Rule MF_01241
Catalytic activity	D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3. HAMAP-Rule MF_01241
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 5/5. HAMAP-Rule MF_01241
Sequence similarities	Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: HAMAP N-acetylneuraminate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glucosamine-6-phosphate deaminase activity Inferred from electronic annotation. Source: HAMAP hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Glucosamine-6-phosphate deaminase HAMAP-Rule MF_01241		PRO_1000066955
Sites
	Active site	63	1	Proton acceptor; for enolization step By similarity
	Active site	129	1	For ring-opening step By similarity
	Active site	131	1	Proton acceptor; for ring-opening step By similarity
	Active site	136	1	For ring-opening step By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q731P4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2E5F5B27B3DFFEF7	FASTA	262	28,971
	10 20 30 40 50 60 MNILVVKTPE ELAEAGYKLI EEVVKTKENP TLGMATGSSP LGIYAEMRKN KLDTSRVTTV 70 80 90 100 110 120 NLDEYVNLPH EDKNSYHYFM QEQLFDHLPF KQTYVPNGMA SDLEEECKRY EGILAANPVD 130 140 150 160 170 180 LQILGIGENG HIGFNEPGTP FNSPTNIVEL TESTRQANLR FFEKEEDVPT HAITMGIGSI 190 200 210 220 230 240 MKAKQILLVA MGSKKAEAVK ELLQGAYSEA CPATVLQRHP NVTVIADQEA LSLCSEAIAD 250 260 EHRQVFTISD LLSDSRVGET AN

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS43023.1.
RefSeq	NP_980415.1. NC_003909.8.
3D structure databases
HSSP	HSSP built from PDB template 2BKX based on UniProtKB O35000.
ProteinModelPortal	Q731P4.
SMR	Q731P4. Positions 1-233.
ModBase	Search...
Protein-protein interaction databases
STRING	222523.BCE_4121.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS43023; AAS43023; BCE_4121.
GeneID	2750382.
KEGG	bca:BCE_4121.
PATRIC	18856889. VBIBacCer118379_3943.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0363.
HOGENOM	HOG000064978.
KO	K02564.
OMA	NDFKPTA.
ProtClustDB	PRK00443.
Enzyme and pathway databases
UniPathway	UPA00629; UER00684.
Family and domain databases
HAMAP	MF_01241. GlcN6P_deamin.
InterPro	IPR006148. Glc/Gal-6P_isomerase. IPR004547. Glucosamine6P_isomerase. IPR018321. Glucosamine6P_isomerase_CS. [Graphical view]
PANTHER	PTHR11280. PTHR11280. 1 hit.
Pfam	PF01182. Glucosamine_iso. 1 hit. [Graphical view]
TIGRFAMs	TIGR00502. nagB. 1 hit.
PROSITE	PS01161. GLC_GALNAC_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGB_BACC1

Accession

Primary (citable) accession number: Q731P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program