Q72XB5 (MURA2_BACC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
EC=2.5.1.7

Alternative name(s):
Enoylpyruvate transferase 2
UDP-N-acetylglucosamine enolpyruvyl transferase 2
Short name=EPT 2

Gene names

Name:	murA2
Ordered Locus Names:	BCE_5463

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231154
Sites
	Active site	117	1	Proton donor By similarity
Amino acid modifications
	Modified residue	117	1	2-(S-cysteinyl)pyruvic acid O-phosphothioketal By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q72XB5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 72F8655C2C0C49DA	FASTA	429	45,866
	10 20 30 40 50 60 MEKLLIEGGR ALNGTIRVSG AKNSAVALIP ATILADTPVT IGGVPNISDV KMLGDLLEEI 70 80 90 100 110 120 GGRVTYGQEE EMVVDPSNMV AMPLPNGKVK KLRASYYLMG AMLGRFKKAV IGLPGGCHLG 130 140 150 160 170 180 PRPIDQHIKG FEALGAHVTN EQGAIYLRAD ELRGARIYLD VVSVGATINI MLAAVRAKGR 190 200 210 220 230 240 TVIENAAKEP EIIDVATLLT SMGARIKGAG TDVIRIDGVD SLHGCHHTII PDRIEAGTYM 250 260 270 280 290 300 ILGAASGGEV TVDNVIPQHL ESVTAKLREA GVQVETNDDQ ITVNGDRRLK VVDIKTLVYP 310 320 330 340 350 360 GFPTDLQQPF TTLLTKAHGT GVVTDTIYGA RFKHIDELRR MNAQIKVEGR SAIVTGPVLL 370 380 390 400 410 420 QGAKVKASDL RAGAALVIAG LMADGITEVT GLEHIDRGYE NIVDKLKGLG ANIWREQMTK QEIEEMKNA

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS44363.1.
RefSeq	NP_981755.1. NC_003909.8.
3D structure databases
ProteinModelPortal	Q72XB5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q72XB5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000028748; EBBACP00000028098; EBBACG00000028739.
GeneID	2748228.
GenomeReviews	Gene locus BCE_5463 in contig AE017194_GR.
KEGG	bca:BCE_5463.
PATRIC	18859526. VBIBacCer118379_5232.
TIGR	BCE_5463.
Phylogenomic databases
eggNOG	COG0766.
GeneTree	EBGT00050000001036.
HOGENOM	HBG482701.
OMA	GATIWRE.
ProtClustDB	PRK12830.
Enzyme and pathway databases
BioCyc	BCER405917:BCE_5463-MONOMER.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00790.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01072. MurA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_BACC1

Accession

Primary (citable) accession number: Q72XB5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program