Q72WY4 (GUAC_BACC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP reductase
EC=1.7.1.7

Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase
Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	BCE_5594

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: EC dihydroorotate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	GMP reductase HAMAP MF_01511		PRO_0000093747
Regions
	Nucleotide binding	204 – 227	24	NADP Potential
Sites
	Active site	175	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q72WY4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 622222621599CB11	FASTA	327	36,113
	10 20 30 40 50 60 MENVFDYEDI QLIPAKCIVN SRSECDTTVT LGKHKFKLPV VPANMQTIID ERIATYLAEN 70 80 90 100 110 120 NYFYIMHRFQ PEKRISFIRD MQSRGLIASI SVGVKADEYE FVQQLAAEHL TPEYITIDIA 130 140 150 160 170 180 HGHSNAVINM IQHIKKHLPE SFVIAGNVGT PEAVRELENA GADATKVGIG PGKVCITKIK 190 200 210 220 230 240 TGFGTGGWQL AALRWCAKAA SKPIIADGGI RTHGDVAKSI RFGATMVMIG SLFAGHEESP 250 260 270 280 290 300 GETIEKDGKL YKEYFGSASE FQKGEKKNVE GKKMFVEHKG SLEDTLIEME QDLQSSISYA 310 320 GGTKLDSIRT VDYVVVKNSI FNGDKVY

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 10987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017194 Genomic DNA. Translation: AAS44494.1.
RefSeq	NP_981886.1. NC_003909.8.
3D structure databases
ProteinModelPortal	Q72WY4.
SMR	Q72WY4. Positions 1-321.
ModBase	Search...
Protein-protein interaction databases
STRING	Q72WY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000025202; EBBACP00000024552; EBBACG00000025193.
GeneID	2748968.
GenomeReviews	Gene locus BCE_5594 in contig AE017194_GR.
KEGG	bca:BCE_5594.
NMPDR	fig|222523.1.peg.5558.
PATRIC	18859778. VBIBacCer118379_5358.
TIGR	BCE_5594.
Phylogenomic databases
eggNOG	COG0516.
GeneTree	EBGT00050000000995.
HOGENOM	HBG298985.
OMA	PDYITID.
ProtClustDB	PRK05458.
Enzyme and pathway databases
BioCyc	BCER405917:BCE_5594-MONOMER.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001295. Dihydroorotate_DH_CS. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00364.
PANTHER	PTHR11911:SF30. PTHR11911:SF30. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_BACC1

Accession

Primary (citable) accession number: Q72WY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program