Q72V31 (PUR2_LEPIC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	LIC_10472

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) [Complete proteome] [HAMAP]

Taxonomic identifier

267671 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Leptospiraceae › Leptospira › Leptospira interrogans › Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151458
Regions
	Domain	113 – 320	208	ATP-grasp
	Nucleotide binding	139 – 200	62	ATP By similarity
Sites
	Metal binding	290	1	Magnesium or manganese By similarity
	Metal binding	292	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q72V31 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D0F4ABC2A9349E68	FASTA	426	46,258
	10 20 30 40 50 60 MQVKLKVLLI GSGGRESAIA FYLRKSVLLS ELKVFPGNGG FPDQELLPPD SFQVLDKNSV 70 80 90 100 110 120 QSFLKQNPFD LIVVGPEDPL VAGFADWAAE LNIPVFGPDS FCAQVEGSKD FAKSLMTEAK 130 140 150 160 170 180 IPTAEYKTFS EYSDSLKYLE SKSIPIVIKA DGLAAGKGVT VATSKEMAQT ALKEIFKDKK 190 200 210 220 230 240 FGSSGNQVVI EEFMEGQEAS IFAISDGDSY FLLPAAQDHK RAFDGDQGPN TGGMGAYCPA 250 260 270 280 290 300 PVISESILQK VKEQIFDPMF DLFRKKGHPY RGLLYAGLMI SPNGEPKVVE FNCRFGDPET 310 320 330 340 350 360 QCVLAMLDGD LLELLYRAST GKIKGIQAAV KKGAAVVVVL AAQGYPDFYE KNIPLNLPET 370 380 390 400 410 420 SGQNVHLFHA GTLKKDGKVF SSGGRILGIV AQGADLKSSV DQAYSFLEKI QAPKTFYRKD IGYRAL

References

[1]

"Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis." Nascimento A.L.T.O., Ko A.I., Martins E.A.L., Monteiro-Vitorello C.B., Ho P.L., Haake D.A., Verjovski-Almeida S., Hartskeerl R.A., Marques M.V., Oliveira M.C., Menck C.F.M., Leite L.C.C., Carrer H., Coutinho L.L., Degrave W.M., Dellagostin O.A., El-Dorry H., Ferro E.S. , Ferro M.I.T., Furlan L.R., Gamberini M., Giglioti E.A., Goes-Neto A., Goldman G.H., Goldman M.H.S., Harakava R., Jeronimo S.M.B., Junqueira-de-Azevedo I.L.M., Kimura E.T., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Marino C.L., Nunes L.R., de Oliveira R.C., Pereira G.G., Reis M.S., Schriefer A., Siqueira W.J., Sommer P., Tsai S.M., Simpson A.J.G., Ferro J.A., Camargo L.E.A., Kitajima J.P., Setubal J.C., Van Sluys M.A. J. Bacteriol. 186:2164-2172(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fiocruz L1-130.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016823 Genomic DNA. Translation: AAS69093.1.
RefSeq	YP_000456.1. NC_005823.1.
3D structure databases
ProteinModelPortal	Q72V31.
ModBase	Search...
Protein-protein interaction databases
STRING	267671.LIC10472.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS69093; AAS69093; LIC_10472.
GeneID	2771801.
KEGG	lic:LIC10472.
PATRIC	22372768. VBILepInt6257_0558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK13789.
Enzyme and pathway databases
BioCyc	LINT267671:GHQI-745-MONOMER.
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_LEPIC

Accession

Primary (citable) accession number: Q72V31

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program