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Q72S00

- NRDJ_LEPIC

UniProt

Q72S00 - NRDJ_LEPIC

Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.By similarity

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Cofactori

    5'-deoxyadenosylcobalamine (coenzyme B12).By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei153 – 1531SubstrateBy similarity
    Binding sitei230 – 2301Substrate; via amide nitrogenBy similarity
    Active sitei482 – 4821Proton acceptorBy similarity
    Active sitei484 – 4841Cysteine radical intermediateBy similarity
    Active sitei486 – 4861Proton acceptorBy similarity

    GO - Molecular functioni

    1. cobalamin binding Source: UniProtKB-KW
    2. cobalt ion binding Source: InterPro
    3. nucleotide binding Source: UniProtKB-KW
    4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    Cobalamin, Cobalt, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLINT267671:GHQI-1584-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleoside-diphosphate reductase NrdJ
    Gene namesi
    Name:nrdJ
    Ordered Locus Names:LIC_11587
    OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
    Taxonomic identifieri267671 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
    ProteomesiUP000007037: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12011201Vitamin B12-dependent ribonucleotide reductasePRO_0000231659Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi199 ↔ 495Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi267671.LIC11587.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni198 – 1992Substrate bindingBy similarity
    Regioni482 – 4865Substrate bindingBy similarity
    Regioni683 – 6875Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    KOiK00525.
    OrthoDBiEOG6H4K5F.

    Family and domain databases

    InterProiIPR013678. RNR_2_N.
    IPR000788. RNR_lg_C.
    IPR013344. RNR_NrdJ/NrdZ.
    IPR024434. TSCPD_dom.
    IPR029072. YebC-like.
    [Graphical view]
    PfamiPF08471. Ribonuc_red_2_N. 1 hit.
    PF02867. Ribonuc_red_lgC. 2 hits.
    PF12637. TSCPD. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    SUPFAMiSSF75625. SSF75625. 1 hit.
    TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q72S00-1 [UniParc]FASTAAdd to Basket

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    MKMNRHFTVP QNGESSTIQW TKRNSKITNP DGSKVFEAND ILVPEDWSQV     50
    AVDILAQKYF RRKGVPKYLK KVQEDGIPEW LQKSIPDTEK LESLKPEDRF 100
    GGETSALEVF HRLAGCWTYW GYKYKYFSDE ESAKIFYDEI VYMLATQMAA 150
    PNSPQWFNTG LNWAYGIDGK SQGHYYVDPS TGKLVKSTSA YEHPQPHACF 200
    IQSVDDDLVN EGGIMDLWVR EARLFKYGSG TGTNFSNLRG ENEPLSGGGK 250
    SSGLMSFLKI GDRAAGAIKS GGTTRRAAKM VCLDVDHPDI ENFIDWKVTE 300
    EKKVASLVTG SMLNNRHLNA IMSACYEMEG EDRFNPKKNS SLKKTIQDAK 350
    KVLIPDNYIK RVIDLARQGY KEILFEELTT DWQSDAYNTV SGQNSNNSIR 400
    LTNEFMAAVE QDQPWNLYFR TEKEKAKVEG RKAKPSQTLR ARELWEKISY 450
    AAWASADPGT QYHTTINEWH TCPEDGPINA SNPCSEYMFL DNTACNLASA 500
    NLQKFVNLET LNFDVEGFRY LCKLWTIILE ISVTMAQFPS KEIAELSYKF 550
    RTLGLGYANL GSVLMVLGIP YDSQQAMAIT GAISSIMHMT AYATSAEMAK 600
    EQGPFVGYAK NQKHMLRVIR NHRRAAYNAP SGDYEGLTIT PIGINPAFCP 650
    SYMLKAAQED ADLALSLGEK YGFRNAQVTV IAPTGTIGLV MDCDTTGIEP 700
    DFALVKFKKL AGGGYFKIIN QSVPYGLKKL GYSPSEIEAI VNYCKGHATL 750
    NGAPVINTQA LKEKGFTNEI LEKVEASLPL AFDINFAFNK FNLGENFLTK 800
    NLGISKEIFD SPGFSLLEHL GFTKEDINKA NDYVCGTMTI ENAPFLKEKD 850
    YPVFDCANKC GKYGKRFLSY ESHIRIMAAA QPFISGAISK TINLPEEAVI 900
    EDIKNAYFLS WKMMIKANAL YRDGSKLSQP LNSVLELLNG IEIDDQEEIR 950
    EATISKDPVQ IAEKIVTKYI SHRRKLPSRR AGYTQKAIVG GHKVYLRTGE 1000
    YEDGQIGEIF IDMHKEGAAF RSLMNAFAIS VSLGLQHGVP LEEYVDAFTF 1050
    FKFEPNGIVS GNKHIKMSTS VIDYIFRELA ITYLGRYDLG QVAPEDLRGD 1100
    EIGSKRATAE SNGQEKETLS SMTAVIEPTP KKEVETISYS QMISKEKPSS 1150
    SPSGISLLEE VKLAKIKGYT GDSCSECGSF EMVRNGSCLK CMSCGSTTGC 1200
    S 1201
    Length:1,201
    Mass (Da):133,918
    Last modified:August 10, 2010 - v2
    Checksum:i062F88B39A9A120C
    GO

    Sequence cautioni

    The sequence AAS70183.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016823 Genomic DNA. Translation: AAS70183.1. Different initiation.
    RefSeqiYP_001546.1. NC_005823.1.

    Genome annotation databases

    EnsemblBacteriaiAAS70183; AAS70183; LIC_11587.
    GeneIDi2772842.
    KEGGilic:LIC11587.
    PATRICi22375523. VBILepInt6257_1927.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016823 Genomic DNA. Translation: AAS70183.1 . Different initiation.
    RefSeqi YP_001546.1. NC_005823.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267671.LIC11587.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS70183 ; AAS70183 ; LIC_11587 .
    GeneIDi 2772842.
    KEGGi lic:LIC11587.
    PATRICi 22375523. VBILepInt6257_1927.

    Phylogenomic databases

    KOi K00525.
    OrthoDBi EOG6H4K5F.

    Enzyme and pathway databases

    BioCyci LINT267671:GHQI-1584-MONOMER.

    Family and domain databases

    InterProi IPR013678. RNR_2_N.
    IPR000788. RNR_lg_C.
    IPR013344. RNR_NrdJ/NrdZ.
    IPR024434. TSCPD_dom.
    IPR029072. YebC-like.
    [Graphical view ]
    Pfami PF08471. Ribonuc_red_2_N. 1 hit.
    PF02867. Ribonuc_red_lgC. 2 hits.
    PF12637. TSCPD. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    SUPFAMi SSF75625. SSF75625. 1 hit.
    TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis."
      Nascimento A.L.T.O., Ko A.I., Martins E.A.L., Monteiro-Vitorello C.B., Ho P.L., Haake D.A., Verjovski-Almeida S., Hartskeerl R.A., Marques M.V., Oliveira M.C., Menck C.F.M., Leite L.C.C., Carrer H., Coutinho L.L., Degrave W.M., Dellagostin O.A., El-Dorry H., Ferro E.S.
      , Ferro M.I.T., Furlan L.R., Gamberini M., Giglioti E.A., Goes-Neto A., Goldman G.H., Goldman M.H.S., Harakava R., Jeronimo S.M.B., Junqueira-de-Azevedo I.L.M., Kimura E.T., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Marino C.L., Nunes L.R., de Oliveira R.C., Pereira G.G., Reis M.S., Schriefer A., Siqueira W.J., Sommer P., Tsai S.M., Simpson A.J.G., Ferro J.A., Camargo L.E.A., Kitajima J.P., Setubal J.C., Van Sluys M.A.
      J. Bacteriol. 186:2164-2172(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fiocruz L1-130.

    Entry informationi

    Entry nameiNRDJ_LEPIC
    AccessioniPrimary (citable) accession number: Q72S00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3