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Q72S00

- NRDJ_LEPIC

UniProt

Q72S00 - NRDJ_LEPIC

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Protein
Vitamin B12-dependent ribonucleotide reductase
Gene
nrdJ, LIC_11587
Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei153 – 1531Substrate By similarity
Binding sitei230 – 2301Substrate; via amide nitrogen By similarity
Active sitei482 – 4821Proton acceptor By similarity
Active sitei484 – 4841Cysteine radical intermediate By similarity
Active sitei486 – 4861Proton acceptor By similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. cobalt ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Enzyme and pathway databases

BioCyciLINT267671:GHQI-1584-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:LIC_11587
OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
Taxonomic identifieri267671 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000007037: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12011201Vitamin B12-dependent ribonucleotide reductase
PRO_0000231659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi199 ↔ 495Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi267671.LIC11587.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 1992Substrate binding By similarity
Regioni482 – 4865Substrate binding By similarity
Regioni683 – 6875Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

KOiK00525.
OrthoDBiEOG6H4K5F.

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
IPR029072. YebC-like.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 2 hits.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF75625. SSF75625. 1 hit.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

Q72S00-1 [UniParc]FASTAAdd to Basket

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MKMNRHFTVP QNGESSTIQW TKRNSKITNP DGSKVFEAND ILVPEDWSQV     50
AVDILAQKYF RRKGVPKYLK KVQEDGIPEW LQKSIPDTEK LESLKPEDRF 100
GGETSALEVF HRLAGCWTYW GYKYKYFSDE ESAKIFYDEI VYMLATQMAA 150
PNSPQWFNTG LNWAYGIDGK SQGHYYVDPS TGKLVKSTSA YEHPQPHACF 200
IQSVDDDLVN EGGIMDLWVR EARLFKYGSG TGTNFSNLRG ENEPLSGGGK 250
SSGLMSFLKI GDRAAGAIKS GGTTRRAAKM VCLDVDHPDI ENFIDWKVTE 300
EKKVASLVTG SMLNNRHLNA IMSACYEMEG EDRFNPKKNS SLKKTIQDAK 350
KVLIPDNYIK RVIDLARQGY KEILFEELTT DWQSDAYNTV SGQNSNNSIR 400
LTNEFMAAVE QDQPWNLYFR TEKEKAKVEG RKAKPSQTLR ARELWEKISY 450
AAWASADPGT QYHTTINEWH TCPEDGPINA SNPCSEYMFL DNTACNLASA 500
NLQKFVNLET LNFDVEGFRY LCKLWTIILE ISVTMAQFPS KEIAELSYKF 550
RTLGLGYANL GSVLMVLGIP YDSQQAMAIT GAISSIMHMT AYATSAEMAK 600
EQGPFVGYAK NQKHMLRVIR NHRRAAYNAP SGDYEGLTIT PIGINPAFCP 650
SYMLKAAQED ADLALSLGEK YGFRNAQVTV IAPTGTIGLV MDCDTTGIEP 700
DFALVKFKKL AGGGYFKIIN QSVPYGLKKL GYSPSEIEAI VNYCKGHATL 750
NGAPVINTQA LKEKGFTNEI LEKVEASLPL AFDINFAFNK FNLGENFLTK 800
NLGISKEIFD SPGFSLLEHL GFTKEDINKA NDYVCGTMTI ENAPFLKEKD 850
YPVFDCANKC GKYGKRFLSY ESHIRIMAAA QPFISGAISK TINLPEEAVI 900
EDIKNAYFLS WKMMIKANAL YRDGSKLSQP LNSVLELLNG IEIDDQEEIR 950
EATISKDPVQ IAEKIVTKYI SHRRKLPSRR AGYTQKAIVG GHKVYLRTGE 1000
YEDGQIGEIF IDMHKEGAAF RSLMNAFAIS VSLGLQHGVP LEEYVDAFTF 1050
FKFEPNGIVS GNKHIKMSTS VIDYIFRELA ITYLGRYDLG QVAPEDLRGD 1100
EIGSKRATAE SNGQEKETLS SMTAVIEPTP KKEVETISYS QMISKEKPSS 1150
SPSGISLLEE VKLAKIKGYT GDSCSECGSF EMVRNGSCLK CMSCGSTTGC 1200
S 1201
Length:1,201
Mass (Da):133,918
Last modified:August 10, 2010 - v2
Checksum:i062F88B39A9A120C
GO

Sequence cautioni

The sequence AAS70183.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016823 Genomic DNA. Translation: AAS70183.1. Different initiation.
RefSeqiYP_001546.1. NC_005823.1.

Genome annotation databases

EnsemblBacteriaiAAS70183; AAS70183; LIC_11587.
GeneIDi2772842.
KEGGilic:LIC11587.
PATRICi22375523. VBILepInt6257_1927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016823 Genomic DNA. Translation: AAS70183.1 . Different initiation.
RefSeqi YP_001546.1. NC_005823.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 267671.LIC11587.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS70183 ; AAS70183 ; LIC_11587 .
GeneIDi 2772842.
KEGGi lic:LIC11587.
PATRICi 22375523. VBILepInt6257_1927.

Phylogenomic databases

KOi K00525.
OrthoDBi EOG6H4K5F.

Enzyme and pathway databases

BioCyci LINT267671:GHQI-1584-MONOMER.

Family and domain databases

InterProi IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
IPR029072. YebC-like.
[Graphical view ]
Pfami PF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 2 hits.
PF12637. TSCPD. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF75625. SSF75625. 1 hit.
TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis."
    Nascimento A.L.T.O., Ko A.I., Martins E.A.L., Monteiro-Vitorello C.B., Ho P.L., Haake D.A., Verjovski-Almeida S., Hartskeerl R.A., Marques M.V., Oliveira M.C., Menck C.F.M., Leite L.C.C., Carrer H., Coutinho L.L., Degrave W.M., Dellagostin O.A., El-Dorry H., Ferro E.S.
    , Ferro M.I.T., Furlan L.R., Gamberini M., Giglioti E.A., Goes-Neto A., Goldman G.H., Goldman M.H.S., Harakava R., Jeronimo S.M.B., Junqueira-de-Azevedo I.L.M., Kimura E.T., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Marino C.L., Nunes L.R., de Oliveira R.C., Pereira G.G., Reis M.S., Schriefer A., Siqueira W.J., Sommer P., Tsai S.M., Simpson A.J.G., Ferro J.A., Camargo L.E.A., Kitajima J.P., Setubal J.C., Van Sluys M.A.
    J. Bacteriol. 186:2164-2172(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fiocruz L1-130.

Entry informationi

Entry nameiNRDJ_LEPIC
AccessioniPrimary (citable) accession number: Q72S00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: August 10, 2010
Last modified: June 11, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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