Reviewed, UniProtKB/Swiss-Prot Q72S00 (NRDJ_LEPIC)

Names and origin

Protein names

Recommended name:
    Vitamin B12-dependent ribonucleotide reductase
    EC=1.17.4.1
Alternative name(s):
    Ribonucleoside-diphosphate reductase nrdJ

Gene names

Name:	nrdJ
Ordered Locus Names:	LIC_11587

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni [Complete proteome] [HAMAP]

Taxonomic identifier

44275 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	1209 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase class-2 family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	Cobalamin Cobalt Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	class II ribonucleotide reductase activity Inferred from electronic annotation. Source: InterPro cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1209	1209	Vitamin B12-dependent ribonucleotide reductase		PRO_0000231659
Sites
	Active site	207	1	By similarity
	Active site	492	1	By similarity
	Active site	503	1	By similarity
	Active site	1202	1	By similarity
	Active site	1208	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q72S00-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 125490B3A5EC2BBB	FASTA	1,209	134,968
	10 20 30 40 50 60 MLWSKKKFMK MNRHFTVPQN GESSTIQWTK RNSKITNPDG SKVFEANDIL VPEDWSQVAV 70 80 90 100 110 120 DILAQKYFRR KGVPKYLKKV QEDGIPEWLQ KSIPDTEKLE SLKPEDRFGG ETSALEVFHR 130 140 150 160 170 180 LAGCWTYWGY KYKYFSDEES AKIFYDEIVY MLATQMAAPN SPQWFNTGLN WAYGIDGKSQ 190 200 210 220 230 240 GHYYVDPSTG KLVKSTSAYE HPQPHACFIQ SVDDDLVNEG GIMDLWVREA RLFKYGSGTG 250 260 270 280 290 300 TNFSNLRGEN EPLSGGGKSS GLMSFLKIGD RAAGAIKSGG TTRRAAKMVC LDVDHPDIEN 310 320 330 340 350 360 FIDWKVTEEK KVASLVTGSM LNNRHLNAIM SACYEMEGED RFNPKKNSSL KKTIQDAKKV 370 380 390 400 410 420 LIPDNYIKRV IDLARQGYKE ILFEELTTDW QSDAYNTVSG QNSNNSIRLT NEFMAAVEQD 430 440 450 460 470 480 QPWNLYFRTE KEKAKVEGRK AKPSQTLRAR ELWEKISYAA WASADPGTQY HTTINEWHTC 490 500 510 520 530 540 PEDGPINASN PCSEYMFLDN TACNLASANL QKFVNLETLN FDVEGFRYLC KLWTIILEIS 550 560 570 580 590 600 VTMAQFPSKE IAELSYKFRT LGLGYANLGS VLMVLGIPYD SQQAMAITGA ISSIMHMTAY 610 620 630 640 650 660 ATSAEMAKEQ GPFVGYAKNQ KHMLRVIRNH RRAAYNAPSG DYEGLTITPI GINPAFCPSY 670 680 690 700 710 720 MLKAAQEDAD LALSLGEKYG FRNAQVTVIA PTGTIGLVMD CDTTGIEPDF ALVKFKKLAG 730 740 750 760 770 780 GGYFKIINQS VPYGLKKLGY SPSEIEAIVN YCKGHATLNG APVINTQALK EKGFTNEILE 790 800 810 820 830 840 KVEASLPLAF DINFAFNKFN LGENFLTKNL GISKEIFDSP GFSLLEHLGF TKEDINKAND 850 860 870 880 890 900 YVCGTMTIEN APFLKEKDYP VFDCANKCGK YGKRFLSYES HIRIMAAAQP FISGAISKTI 910 920 930 940 950 960 NLPEEAVIED IKNAYFLSWK MMIKANALYR DGSKLSQPLN SVLELLNGIE IDDQEEIREA 970 980 990 1000 1010 1020 TISKDPVQIA EKIVTKYISH RRKLPSRRAG YTQKAIVGGH KVYLRTGEYE DGQIGEIFID 1030 1040 1050 1060 1070 1080 MHKEGAAFRS LMNAFAISVS LGLQHGVPLE EYVDAFTFFK FEPNGIVSGN KHIKMSTSVI 1090 1100 1110 1120 1130 1140 DYIFRELAIT YLGRYDLGQV APEDLRGDEI GSKRATAESN GQEKETLSSM TAVIEPTPKK 1150 1160 1170 1180 1190 1200 EVETISYSQM ISKEKPSSSP SGISLLEEVK LAKIKGYTGD SCSECGSFEM VRNGSCLKCM SCGSTTGCS

References

[1]

"Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis." Nascimento A.L.T.O., Ko A.I., Martins E.A.L., Monteiro-Vitorello C.B., Ho P.L., Haake D.A., Verjovski-Almeida S., Hartskeerl R.A., Marques M.V., Oliveira M.C., Menck C.F.M., Leite L.C.C., Carrer H., Coutinho L.L., Degrave W.M., Dellagostin O.A., El-Dorry H., Ferro E.S. , Ferro M.I.T., Furlan L.R., Gamberini M., Giglioti E.A., Goes-Neto A., Goldman G.H., Goldman M.H.S., Harakava R., Jeronimo S.M.B., Junqueira-de-Azevedo I.L.M., Kimura E.T., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Marino C.L., Nunes L.R., de Oliveira R.C., Pereira G.G., Reis M.S., Schriefer A., Siqueira W.J., Sommer P., Tsai S.M., Simpson A.J.G., Ferro J.A., Camargo L.E.A., Kitajima J.P., Setubal J.C., Van Sluys M.A. J. Bacteriol. 186:2164-2172(2004) [PubMed: 15028702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fiocruz L1-130.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016823 Genomic DNA. Translation: AAS70183.1.
RefSeq	YP_001546.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2772842.
GenomeReviews	Gene locus LIC_11587 in contig AE016823_GR.
KEGG	lic:LIC11587.
NMPDR	fig|267671.1.peg.1546.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q72S00.
OMA	LDYVFRE.
Enzyme and pathway databases
BioCyc	LINT267671:LIC_11587-MON.
Family and domain databases
InterPro	IPR013344. RDP_Rdtase_AdoCbl-dep. IPR000788. Ribncl_red_lg_C. IPR013678. Ribonuc_red_2_N. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF08471. Ribonuc_red_2_N. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02504. NrdJ_Z. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NRDJ_LEPIC

Accession

Primary (citable) accession number: Q72S00

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)