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Q72RG8 (BIOB_LEPIC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence AAS70366.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351Biotin synthase HAMAP-Rule MF_01694
PRO_0000381447

Sites

Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1071Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1391Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1991Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2691Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q72RG8 [UniParc].

Last modified August 10, 2010. Version 2.
Checksum: D13A68C967049BCF

FASTA35139,251
        10         20         30         40         50         60 
MLKTSEKIFS ETPSIITKEE GLKILNGVIP LTTCLDKAFQ ERNRYFENKV RIHILDNIKN 

        70         80         90        100        110        120 
GYCPEDCGYC AQRKNANSGV QEYPMKSEQE IYEDAVQAKK NGAYRFCMVT SGTGPNRLTT 

       130        140        150        160        170        180 
EKLASTIQRI TDELNMKVCL SAGLLDIEKA QVLKEAGLDR YNHNLNTSEN HYSEICDTHT 

       190        200        210        220        230        240 
YLQRAQTLDS VSKAGIGMCS GVIVGMGESF QDIVDVAFQL KSFRVISIPV NFFIPVKGHT 

       250        260        270        280        290        300 
IKNPSVLTPE LCVRILCMFR LINPDSEIRI AAGREGHLRS LSATALFAAN SLFSSGYLNV 

       310        320        330        340        350 
KGSEILETVA MIRDAGFVPE LSNGEILPEN FGTESFYSEK NFPELYKFKK F 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016823 Genomic DNA. Translation: AAS70366.1. Different initiation.
RefSeqYP_001729.1. NC_005823.1.

3D structure databases

ProteinModelPortalQ72RG8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267671.LIC11777.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS70366; AAS70366; LIC_11777.
GeneID2772393.
KEGGlic:LIC11777.
PATRIC22375951. VBILepInt6257_2141.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01012.
OrthoDBEOG622PMP.
ProtClustDBCLSK574240.

Enzyme and pathway databases

BioCycLINT267671:GHQI-1773-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_LEPIC
AccessionPrimary (citable) accession number: Q72RG8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 10, 2010
Last modified: February 19, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways