Reviewed, UniProtKB/Swiss-Prot Q72Q29 (HPPA_LEPIC)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	LIC_12285

Organism

Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni [Complete proteome] [HAMAP]

Taxonomic identifier

44275 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217017
Regions
	Transmembrane	3 – 23	21	Potential
	Transmembrane	64 – 84	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	144 – 164	21	Potential
	Transmembrane	174 – 194	21	Potential
	Transmembrane	276 – 296	21	Potential
	Transmembrane	313 – 333	21	Potential
	Transmembrane	346 – 366	21	Potential
	Transmembrane	387 – 407	21	Potential
	Transmembrane	429 – 449	21	Potential
	Transmembrane	483 – 503	21	Potential
	Transmembrane	514 – 534	21	Potential
	Transmembrane	586 – 606	21	Potential
	Transmembrane	608 – 628	21	Potential
	Transmembrane	680 – 700	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q72Q29-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9369E135382D96BC	FASTA	704	73,106
	10 20 30 40 50 60 MNSVTIIIAM SILAIVTAVV YTLKVTSIKV GTLGGNEKET KKLLEISSAI SEGAMAFLVR 70 80 90 100 110 120 EYKVISLFIA FMAVLIVLLL DNPGSEGFND GIYTAIAFVS GALISCISGF IGMKIATAGN 130 140 150 160 170 180 VRTAEAAKSS MAKAFRVAFD SGAVMGFGLV GLAILGMIVL FLVFTGMYPG VEKHFLMESL 190 200 210 220 230 240 AGFGLGGSAV ALFGRVGGGI YTKAADVGAD LVGKVEKGIP EDDPRNPATI ADNVGDNVGD 250 260 270 280 290 300 VAGMGADLFG SCAEATCAAL VIGATASALS GSVDALLYPL LISAFGIPAS ILTSFLARVK 310 320 330 340 350 360 EDGNVESALK VQLWVSTLLV AGIMYFVTKT FMVDSFEIAG KTITKWDVYI SMVVGLFSGM 370 380 390 400 410 420 FIGIVTEYYT SHSYKPVREV AEASNTGAAT NIIYGLSLGY HSSVIPVILL VITIVTANLL 430 440 450 460 470 480 AGMYGIAIAA LGMISTIAIG LTIDAYGPVS DNAGGIAEMA ELGKEVRDRT DTLDAAGNTT 490 500 510 520 530 540 AAIGKGFAIG SAALTSLALF AAFITRTHTT SLEVLNAEVF GGLMFGAMLP FLFTAMTMKS 550 560 570 580 590 600 VGKAAVDMVE EVRKQFKEIP GIMEGKNKPD YKRCVDISTS AALREMILPG LLVLLTPILV 610 620 630 640 650 660 GYLFGVKTLA GVLAGALVAG VVLAISAANS GGGWDNAKKY IEKKAGGKGS DQHKAAVVGD 670 680 690 700 TVGDPFKDTS GPSINILIKL MAITSLVFAE FFVQQGGLIF KIFH

References

[1]

"Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis." Nascimento A.L.T.O., Ko A.I., Martins E.A.L., Monteiro-Vitorello C.B., Ho P.L., Haake D.A., Verjovski-Almeida S., Hartskeerl R.A., Marques M.V., Oliveira M.C., Menck C.F.M., Leite L.C.C., Carrer H., Coutinho L.L., Degrave W.M., Dellagostin O.A., El-Dorry H., Ferro E.S. , Ferro M.I.T., Furlan L.R., Gamberini M., Giglioti E.A., Goes-Neto A., Goldman G.H., Goldman M.H.S., Harakava R., Jeronimo S.M.B., Junqueira-de-Azevedo I.L.M., Kimura E.T., Kuramae E.E., Lemos E.G.M., Lemos M.V.F., Marino C.L., Nunes L.R., de Oliveira R.C., Pereira G.G., Reis M.S., Schriefer A., Siqueira W.J., Sommer P., Tsai S.M., Simpson A.J.G., Ferro J.A., Camargo L.E.A., Kitajima J.P., Setubal J.C., Van Sluys M.A. J. Bacteriol. 186:2164-2172(2004) [PubMed: 15028702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fiocruz L1-130.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016823 Genomic DNA. Translation: AAS70856.1.
RefSeq	YP_002219.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2770052.
GenomeReviews	Gene locus LIC_12285 in contig AE016823_GR.
KEGG	lic:LIC12285.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q72Q29.
OMA	VFSAMAM.
Enzyme and pathway databases
BioCyc	LINT267671:LIC_12285-MON.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_LEPIC

Accession

Primary (citable) accession number: Q72Q29

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)