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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei557Aminoacyl-adenylateUniRule annotation1
Binding sitei601ATPUniRule annotation1
Metal bindingi889ZincUniRule annotation1
Metal bindingi892ZincUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LIC_12400
OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
Taxonomic identifieri267671 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesLeptospiralesLeptospiraceaeLeptospira
Proteomesi
  • UP000007037 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984091 – 914Isoleucine--tRNA ligaseAdd BLAST914

Proteomic databases

PaxDbiQ72PR7

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi267671.LIC12400

Structurei

3D structure databases

ProteinModelPortaliQ72PR7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi64 – 74"HIGH" regionAdd BLAST11
Motifi598 – 602"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q72PR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETQKENPY SSTVLLPKTD FPMKADLAKR EPAQIQSWKS GQIFRKMKEQ
60 70 80 90 100
RKGKKEFVLH DGPPYANGNF HLGHSLNKIV KDIIIKSKSL AGFYADMIPG
110 120 130 140 150
WDCHGLPIEV QVLKNLGKKA RETGPEELRR LCRNYAEEFV GKQGEDLSRF
160 170 180 190 200
LCFWEEDRIY KTMSPGFEAK IVEVFGELFQ KGYVYRGKKP VYWSIDLATA
210 220 230 240 250
HAEAEIEYYP HVSPSIYVKF PIVGEQKKFC LIWTTTPWTL PANLGICFNR
260 270 280 290 300
KIEYSFFKTE SGEELILADG LAESVTSTTG VSLNKVKSIS SDELSVLKFQ
310 320 330 340 350
HPFMDRISIS LFGDHVTLDA GTGCVHTAPG HGQDDYKVGL AAGLEPFSPV
360 370 380 390 400
DDYGKYTDEF PLMQGKKVFD ANPEIIQLLK DKGLLLHHSE FEHSYPHSWR
410 420 430 440 450
SKKPLIFRAT PQWFFKMDFN ELREKSLSAI DGVQWIPSWG ITRIRSMVET
460 470 480 490 500
RPDWCLSRQR NWGVPIPAFT CESCGQTHID DVSIQFFTKM VREKGIEIWY
510 520 530 540 550
SEKAADLLPP GTKCGKCGSD SFKKGNDILD VWFDSGVSSF AVLGERKDEP
560 570 580 590 600
PADLYFEGSD QHRGWFQSSL WPSMALRGIP PYKTVLTHGY VLDEKGHPMS
610 620 630 640 650
KSLGNGIDPT ADVIQIYGAD ILRLWVSSLD FRDDIKVGKE SLKIVSEQYR
660 670 680 690 700
KIRNTFRYLL GNLDGHTSEQ NLPFEELEEL DLFYLSKLSQ FAEDVIANYE
710 720 730 740 750
AYQFHQIYQK LILFCTVTLS QDYFDMIRDR MYCDARNSKT RKSSATALQY
760 770 780 790 800
ILETLCILTA PILSFTAEEV WVSNGKKDSV FLQNFPDLKF WKNQNLEEKF
810 820 830 840 850
ESVLQVREVV QKALEIARQE NKLGKSLEAG LEIVSKNGTN LTKILPKETL
860 870 880 890 900
EILFVVSQVH EKNPGLDVLS FYENEKFSVK VLKPVQVECP RCWRHTEDIS
910
KEGDLCGRCK SVVG
Length:914
Mass (Da):104,366
Last modified:July 5, 2004 - v1
Checksum:i31A0AC713E060F2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016823 Genomic DNA Translation: AAS70969.1
RefSeqiWP_000005312.1, NC_005823.1

Genome annotation databases

EnsemblBacteriaiAAS70969; AAS70969; LIC_12400
KEGGilic:LIC_12400

Similar proteinsi

Entry informationi

Entry nameiSYI_LEPIC
AccessioniPrimary (citable) accession number: Q72PR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health