true2013-07-242024-03-27116SIGA_THET2Cloning and characterization in Escherichia coli of the gene encoding the principal sigma factor of an extreme thermophile, Thermus thermophilus.Nishiyama M.Kobashi N.Tanaka K.Takahashi H.Tanokura M.doi:10.1111/j.1574-6968.1999.tb13467.x1999FEMS Microbiol. Lett.172179-186NUCLEOTIDE SEQUENCE [GENOMIC DNA]PROTEIN SEQUENCE OF 1-10GENE NAMEATCC BAA-163 / DSM 7039 / HB27The genome sequence of the extreme thermophile Thermus thermophilus.Henne A.Brueggemann H.Raasch C.Wiezer A.Hartsch T.Liesegang H.Johann A.Lienard T.Gohl O.Martinez-Arias R.Jacobi C.Starkuviene V.Schlenczeck S.Dencker S.Huber R.Klenk H.-P.Kramer W.Merkl R.Gottschalk G.Fritz H.-J.doi:10.1038/nbt9562004Nat. Biotechnol.22547-553NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution.Vassylyev D.G.Sekine S.Laptenko O.Lee J.Vassylyeva M.N.Borukhov S.Yokoyama S.doi:10.1038/nature7522002Nature417712-719X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH RPOA; RPOB; RPOC AND RPOZDOMAINMOTIFSUBUNITStructural basis for transcription regulation by alarmone ppGpp.Artsimovitch I.Patlan V.Sekine S.Vassylyeva M.N.Hosaka T.Ochi K.Yokoyama S.Vassylyev D.G.doi:10.1016/s0092-8674(04)00401-52004Cell117299-310X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) IN COMPLEX WITH RPOA; RPOB; RPOC AND RPOZSUBUNIT2.60F/P=1-4232.70F/P=1-423BacteriaSigma70_r4Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domainSigma70_RpoD_SigARNA_pol_sigma-70_domRNA_pol_sigma70RNA_pol_sigma70_r1_2RNA_pol_sigma70_r2RNA_pol_sigma70_r3RNA_pol_sigma70_r4RNA_pol_sigma_r2RNA_pol_sigma_r3/r4-likeRNA_pol_sigma_RpoD_CSigma70_RpoDWH-like_DNA-bd_sfRpoD_Ctermsigma70-ECFRNA POLYMERASE SIGMA FACTOR RPORNA POLYMERASE SIGMA FACTOR SIGF, CHLOROPLASTICSigma70_r1_2Sigma70_r2Sigma70_r3Sigma70_r4SIGMA70FCTSigma2 domain of RNA polymerase sigma factorsSigma3 and sigma4 domains of RNA polymerase sigma factorsSIGMA70_2RNA polymerase sigma factor SigAsigArpoDTT_C0164Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.Contains 4 domains, connected by flexible linkers. In the active conformation, the domains are in an extended conformation, each making extensive interactions with the RNA polymerase catalytic core (PubMed:12000971).In the autoinhibited state, sigma-70 factor domain-1 packs closely together with sigma-70 factor domains-2 and -4, contrary to the extended conformation that is seen when the protein is part of the RNA polymerase holoenzyme.The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble (By similarity). The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (PubMed:12000971).The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core (By similarity).Belongs to the sigma-70 factor family. RpoD/SigA subfamily.RNA polymerase sigma factor SigA484941423H-T-H motif383402Disordered76Sigma-70 factor domain-178113Sigma-70 factor domain-2187257Sigma-70 factor domain-3266344Sigma-70 factor domain-4357409Interaction with polymerase core subunit RpoC211214Acidic residues196990971201241361391431441461481501541651681901921992032052092262352532552582622782792812822842892953013113153183223243253283293313363383423593633743853883893923944144174202004-07-0514849416f68e5b36e75beed35e498af5b7524aMKKSKRKNAQAQEAQETEVLVQEEAEELPEFPEGEPDPDLEDPDLALEDDLLDLPEEGEGLDLEEEEEDLPIPKISTSDPVRQYLHEIGQVPLLTLEEEVELARKVEEGMEAIKKLSEITGLDPDLIREVVRAKILGSARVRHIPGLKETLDPKTVEEIDQKLKSLPKEHKRYLHIAREGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKFEYKRRFKFSTYATWWIRQAINRAIADQARTIRIPVHMVETINKLSRTARQLQQELGREPTYEEIAEAMGPGWDAKRVEETLKIAQEPVSLETPIGDEKDSFYGDFIPDEHLPSPVDAATQSLLSEELEKALSKLSEREAMVLKLRKGLIDGREHTLEEVGAFFGVTRERIRQIENKALRKLKYHESRTRKLRDFLDtruetruetruetrue