Q72KE8 (IF2_THET2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2

Gene names

Name:	infB
Ordered Locus Names:	TT_C0347

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the IF-2 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Translation initiation factor IF-2 HAMAP-Rule MF_00100		PRO_0000228255
Regions
	Nucleotide binding	80 – 87	8	GTP By similarity
	Nucleotide binding	126 – 130	5	GTP By similarity
	Nucleotide binding	180 – 183	4	GTP By similarity
	Region	74 – 222	149	G-domain HAMAP-Rule MF_00100

Sequences

	Sequence		Length	Mass (Da)
	Q72KE8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7FA1269583DB969F	FASTA	571	63,178
	10 20 30 40 50 60 MAKVRIYQLA KELGMETQEL LELLDQMGVA YKSHASTLEE KDAEAVRELV KEQRGLQEKL 70 80 90 100 110 120 AEEERRKSLP RRPPVVVIMG HVDHGKTTLL DYLRKSRIAE KEAGGITQHV GAFEVKTPQG 130 140 150 160 170 180 TVVFIDTPGH EAFTTIRQRG AKVADIAVIV IAADDGIMPQ TEEAIAHAKA AGAKLIFAIN 190 200 210 220 230 240 KIDLPQADPE KVKRQLMERG FVPEEYGGDA IVIPISAKTG QGVQDLLEMI LLLAELEDYR 250 260 270 280 290 300 ADPNAEPRGV ILESKLDKQA GIIANMLVQE GTFRVGDYVV AGEAYGRIRA MMDADGNQRK 310 320 330 340 350 360 EAGPGSAVQV LGFQELPHAG DVVEWVPDLE AAKEIAEERK EERKAREEEE KARRPRTMAE 370 380 390 400 410 420 LLRAMQEEGR KELNLILRAD TQGSLEAIQH ILARESTEDV KINILLAQVG APTESDVLLA 430 440 450 460 470 480 QTANAAILAF GVNPPGSVKK KAEEKGVLLK TFRIIYDLVD EVRNMVKGQR EPQYKEEVLG 490 500 510 520 530 540 QAEVRAIFRL PTGKQVAGCM VTQGRIPRNA EVRVLRDGQV IWQGRIASLK RFKEDVREVA 550 560 570 QGYECGIGLD GFDDFREGDV IEAFQMVEVP A

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB27 / ATCC BAA-163 / DSM 7039.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017221 Genomic DNA. Translation: AAS80695.1.
RefSeq	YP_004322.1. NC_005835.1.
3D structure databases
ProteinModelPortal	Q72KE8.
ModBase	Search...
Protein-protein interaction databases
STRING	262724.TTC0347.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS80695; AAS80695; TT_C0347.
GeneID	2776475.
KEGG	tth:TTC0347.
PATRIC	23951091. VBITheThe54392_0345.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0532.
KO	K02519.
OMA	HIGAFTV.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
HAMAP	MF_00100_B. IF-2_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR005225. Small_GTP-bd_dom. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 2 hits. PF11987. IF-2. 1 hit. PF04760. IF2_N. 1 hit. [Graphical view]
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2_THET2

Accession

Primary (citable) accession number: Q72KE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program