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Protein

N(1)-aminopropylagmatine ureohydrolase

Gene

TT_C0764

Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of polyamines which are thought to support the growth of thermophilic microorganisms under high-temperature conditions. It seems that long-chain and branched-chain of polyamines effectively stabilize DNA and RNA, respectively. Catalyzes the decarboxylation of N1-(3-aminopropyl)agmatine to yield spermidine and urea. Does not act on agmatine.1 Publication

Miscellaneous

The biosynthetic pathway, by which spermidine is synthesized from agmatine via N1-aminopropylagmatine has been characterized in the hyperthermophilic archaeon Pyrococcus kodakarensis and the thermophilic Gram-negative bacterium Thermus thermophilus.1 Publication

Catalytic activityi

N1-aminopropylagmatine + H2O = spermidine + urea.1 Publication

Cofactori

Mn2+PROSITE-ProRule annotationNote: Binds 2 manganese ions per subunit.PROSITE-ProRule annotation

Pathwayi: spermidine biosynthesis

This protein is involved in the pathway spermidine biosynthesis, which is part of Amine and polyamine biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Manganese 1PROSITE-ProRule annotation1
Metal bindingi128Manganese 1PROSITE-ProRule annotation1
Metal bindingi128Manganese 2PROSITE-ProRule annotation1
Metal bindingi130Manganese 2PROSITE-ProRule annotation1
Metal bindingi132Manganese 1PROSITE-ProRule annotation1
Sitei144Important for catalytic activityBy similarity1
Metal bindingi210Manganese 1PROSITE-ProRule annotation1
Metal bindingi210Manganese 2PROSITE-ProRule annotation1
Metal bindingi212Manganese 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processPolyamine biosynthesis, Spermidine biosynthesis
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciTTHE262724:G1G0N-783-MONOMER
UniPathwayiUPA00248

Names & Taxonomyi

Protein namesi
Recommended name:
N(1)-aminopropylagmatine ureohydrolase (EC:3.5.3.24)
Alternative name(s):
Protein SpeB homolog
Gene namesi
Ordered Locus Names:TT_C0764
OrganismiThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Taxonomic identifieri262724 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000592 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004242131 – 293N(1)-aminopropylagmatine ureohydrolaseAdd BLAST293

Interactioni

Protein-protein interaction databases

STRINGi262724.TTC0764

Structurei

3D structure databases

ProteinModelPortaliQ72JK8
SMRiQ72JK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arginase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CYW Bacteria
COG0010 LUCA
KOiK01480
OMAiDTYFGAE

Family and domain databases

InterProiView protein in InterPro
IPR005925 Agmatinase-rel
IPR006035 Ureohydrolase
IPR023696 Ureohydrolase_dom_sf
PfamiView protein in Pfam
PF00491 Arginase, 1 hit
PIRSFiPIRSF036979 Arginase, 1 hit
SUPFAMiSSF52768 SSF52768, 1 hit
TIGRFAMsiTIGR01230 agmatinase, 1 hit
PROSITEiView protein in PROSITE
PS51409 ARGINASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q72JK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLVFGEKDA PYEEARVVVL PVPYDLSLSF LPGARRGPEA ILLASRELEP
60 70 80 90 100
FLLELGAAPE EVGIHAAEPV PWVAGMAEES HRLIREEALK HLRAGKWLVA
110 120 130 140 150
LGGDHSVTHP LVQAHREALG EFSLLHVDAH ADLYPEWQGS VYSHASPFYR
160 170 180 190 200
LLTEGFPLVQ VGIRAMDRDS LRLARKRGVA LFPAHRIHRE GLPLDEILEA
210 220 230 240 250
LGKRVYISLD FDALDPSLMP SVGTPLPGGL SYRQVVDLLE AVFREKEVVG
260 270 280 290
MDFVELSPNG QFHAEMTAAQ LVYHAIGLKG LQAGWLSREV DHI
Length:293
Mass (Da):32,454
Last modified:July 5, 2004 - v1
Checksum:i5C7CB462E7C57B5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017221 Genomic DNA Translation: AAS81110.1
RefSeqiWP_011173199.1, NC_005835.1

Genome annotation databases

EnsemblBacteriaiAAS81110; AAS81110; TT_C0764
KEGGitth:TT_C0764

Similar proteinsi

Entry informationi

Entry nameiSPEBH_THET2
AccessioniPrimary (citable) accession number: Q72JK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health