Q72JJ8 (PNP_THET2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8

Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	TT_C0774

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP-Rule MF_01595
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: HAMAP polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 713	713	Polyribonucleotide nucleotidyltransferase HAMAP-Rule MF_01595		PRO_0000329916
Regions
	Domain	565 – 631	67	KH
	Domain	633 – 701	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q72JJ8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2979859D9AC5EA82	FASTA	713	78,193
	10 20 30 40 50 60 MEGTPNVPQA HRYELTLAGR PLVLETGKYA KQASGSVLVR YADTVVLATA QASETPVEAD 70 80 90 100 110 120 FLPLTVEFEE RHYAVGKIPG SFMRREGRPG EKAILSARMT DRPIRPLFPK GFRHEVQIIV 130 140 150 160 170 180 TVLSADQKNP PDILGPIAAS AALMLSDIPW EGPIAAVRVG LIGGSFVLNP TLQELEESQL 190 200 210 220 230 240 DLVVAGSKEA ILMVEAEAGE VDEETLVQAL EFAHKEMQPI LELQEAMARE LAKPKMAWTP 250 260 270 280 290 300 PESLPEEEKE ALYRLALERG LSQVLQTASK GERSRALAEF AERLIAEALP KGEDGTPDEG 310 320 330 340 350 360 KKPLYESAFD EVVRRELRRL VLEEGKRADG RGPKDLRPIW IEVDVLPRAH GSAVFTRGET 370 380 390 400 410 420 QVLGTVTLGT GRDEQILDDL GIDETEKFLV HYNFPPFSTG EVRRLRGVSR REVGHGNLAK 430 440 450 460 470 480 RALKAVMPKE EDFPYTIRVV GDVLESNGSS SMATVCAGCL ALMDAGVPIR APVAGVAMGL 490 500 510 520 530 540 VWEENRAVIL TDILGLEDAL GDMDFKVAGT RKGVTALQMD NKVGGLPREV LKEALLQARE 550 560 570 580 590 600 ARLKILDLME TVLPAPRPEL KPFAPRILSL KVPVEKIGLV IGPGGKNVRA LEELGVEVDI 610 620 630 640 650 660 EEDGTVRIYS SDLNAALEAK KRIEDLTREA KVGEIYEGTV TRITPFGAFI SLFPGTEGLL 670 680 690 700 710 HISQIAPGRV ERVEDHLKVG DVIKVKVHRI DERGKIDLIR PELEGKIPPR RRK

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB27 / ATCC BAA-163 / DSM 7039.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017221 Genomic DNA. Translation: AAS81120.1.
RefSeq	YP_004747.1. NC_005835.1.
3D structure databases
HSSP	HSSP built from PDB template 1SRO based on UniProtKB P05055.
ProteinModelPortal	Q72JJ8.
ModBase	Search...
Protein-protein interaction databases
STRING	262724.TTC0774.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS81120; AAS81120; TT_C0774.
GeneID	2774654.
KEGG	tth:TTC0774.
PATRIC	23951953. VBITheThe54392_0771.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1185.
KO	K00962.
OMA	YTMRVVS.
ProtClustDB	PRK11824.
Family and domain databases
Gene3D	1.10.10.400. 1 hit. 2.40.50.140. 1 hit.
HAMAP	MF_01595. PNPase.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH_dom. IPR004088. KH_dom_type_1. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR020568. Ribosomal_S5_D2-typ_fold. IPR022967. RNA-binding_domain_S1. [Graphical view]
PANTHER	PTHR11252. PTHR11252. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF46915. 3_ExoRNase. 1 hit. SSF55666. 3_ExoRNase. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMs	TIGR03591. polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_THET2

Accession

Primary (citable) accession number: Q72JJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program