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Q72JJ8 (PNP_THET2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase

EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:TT_C0774
OrganismThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]
Taxonomic identifier262724 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length713 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP-Rule MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_01595

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01595

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 713713Polyribonucleotide nucleotidyltransferase HAMAP-Rule MF_01595
PRO_0000329916

Regions

Domain565 – 63167KH
Domain633 – 70169S1 motif

Sites

Metal binding4981Magnesium By similarity
Metal binding5041Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q72JJ8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 2979859D9AC5EA82

FASTA71378,193
        10         20         30         40         50         60 
MEGTPNVPQA HRYELTLAGR PLVLETGKYA KQASGSVLVR YADTVVLATA QASETPVEAD 

        70         80         90        100        110        120 
FLPLTVEFEE RHYAVGKIPG SFMRREGRPG EKAILSARMT DRPIRPLFPK GFRHEVQIIV 

       130        140        150        160        170        180 
TVLSADQKNP PDILGPIAAS AALMLSDIPW EGPIAAVRVG LIGGSFVLNP TLQELEESQL 

       190        200        210        220        230        240 
DLVVAGSKEA ILMVEAEAGE VDEETLVQAL EFAHKEMQPI LELQEAMARE LAKPKMAWTP 

       250        260        270        280        290        300 
PESLPEEEKE ALYRLALERG LSQVLQTASK GERSRALAEF AERLIAEALP KGEDGTPDEG 

       310        320        330        340        350        360 
KKPLYESAFD EVVRRELRRL VLEEGKRADG RGPKDLRPIW IEVDVLPRAH GSAVFTRGET 

       370        380        390        400        410        420 
QVLGTVTLGT GRDEQILDDL GIDETEKFLV HYNFPPFSTG EVRRLRGVSR REVGHGNLAK 

       430        440        450        460        470        480 
RALKAVMPKE EDFPYTIRVV GDVLESNGSS SMATVCAGCL ALMDAGVPIR APVAGVAMGL 

       490        500        510        520        530        540 
VWEENRAVIL TDILGLEDAL GDMDFKVAGT RKGVTALQMD NKVGGLPREV LKEALLQARE 

       550        560        570        580        590        600 
ARLKILDLME TVLPAPRPEL KPFAPRILSL KVPVEKIGLV IGPGGKNVRA LEELGVEVDI 

       610        620        630        640        650        660 
EEDGTVRIYS SDLNAALEAK KRIEDLTREA KVGEIYEGTV TRITPFGAFI SLFPGTEGLL 

       670        680        690        700        710 
HISQIAPGRV ERVEDHLKVG DVIKVKVHRI DERGKIDLIR PELEGKIPPR RRK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017221 Genomic DNA. Translation: AAS81120.1.
RefSeqYP_004747.1. NC_005835.1.

3D structure databases

ProteinModelPortalQ72JJ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262724.TTC0774.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS81120; AAS81120; TT_C0774.
GeneID2774654.
KEGGtth:TTC0774.
PATRIC23951953. VBITheThe54392_0771.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1185.
KOK00962.
OMALDFFPLT.
OrthoDBEOG6WT8CC.
ProtClustDBPRK11824.

Enzyme and pathway databases

BioCycTTHE262724:GCAT-786-MONOMER.

Family and domain databases

Gene3D1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPMF_01595. PNPase.
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERPTHR11252. PTHR11252. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsTIGR03591. polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_THET2
AccessionPrimary (citable) accession number: Q72JJ8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families