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Protein

Ribose-5-phosphate isomerase A

Gene

rpiA

Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the first step of the non-oxidative branch of the pentose phosphate pathway. It catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate. Can also act on D-ribose-5-diphosphate and D-ribose-5-triphosphate as substrate.1 Publication

Catalytic activityi

D-ribose 5-phosphate = D-ribulose 5-phosphate.UniRule annotation

Kineticsi

Kcat is 1072 sec(-1) for ribose-5-phosphate.

  1. KM=1.63 mM for ribose-5-phosphate (at 50 degrees Celsius)1 Publication

    Pathwayi: pentose phosphate pathway

    This protein is involved in step 1 of the subpathway that synthesizes D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage).UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Ribose-5-phosphate isomerase A (rpiA)
    This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei108 – 1081Proton acceptorUniRule annotation1 Publication
    Binding sitei126 – 1261Substrate

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciTTHE262724:GCAT-944-MONOMER.
    UniPathwayiUPA00115; UER00412.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribose-5-phosphate isomerase AUniRule annotation (EC:5.3.1.6UniRule annotation)
    Alternative name(s):
    Phosphoriboisomerase AUniRule annotation
    Short name:
    PRIUniRule annotation
    Gene namesi
    Name:rpiAUniRule annotation
    Ordered Locus Names:TT_C0932
    OrganismiThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
    Taxonomic identifieri262724 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    Proteomesi
    • UP000000592 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227Ribose-5-phosphate isomerase APRO_0000158486Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation1 Publication

    Protein-protein interaction databases

    STRINGi262724.TTC0932.

    Structurei

    Secondary structure

    1
    227
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 1712Combined sources
    Beta strandi25 – 284Combined sources
    Helixi32 – 4615Combined sources
    Beta strandi54 – 596Combined sources
    Helixi60 – 689Combined sources
    Beta strandi81 – 866Combined sources
    Beta strandi89 – 924Combined sources
    Helixi93 – 953Combined sources
    Beta strandi96 – 983Combined sources
    Helixi105 – 1139Combined sources
    Beta strandi115 – 1239Combined sources
    Helixi124 – 1263Combined sources
    Beta strandi129 – 1313Combined sources
    Beta strandi136 – 1405Combined sources
    Helixi145 – 1539Combined sources
    Turni154 – 1563Combined sources
    Beta strandi159 – 1613Combined sources
    Beta strandi176 – 1805Combined sources
    Helixi188 – 1969Combined sources
    Beta strandi201 – 2077Combined sources
    Beta strandi212 – 2187Combined sources
    Beta strandi221 – 2255Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UJ4X-ray1.80A1-227[»]
    1UJ5X-ray2.00A1-227[»]
    1UJ6X-ray1.74A1-227[»]
    ProteinModelPortaliQ72J47.
    SMRiQ72J47. Positions 3-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ72J47.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni30 – 334Substrate binding
    Regioni86 – 894Substrate binding
    Regioni99 – 1046Substrate binding

    Sequence similaritiesi

    Belongs to the ribose 5-phosphate isomerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105E66. Bacteria.
    COG0120. LUCA.
    KOiK01807.
    OMAiGACHVQE.
    OrthoDBiEOG67MF61.

    Family and domain databases

    HAMAPiMF_00170. Rib_5P_isom_A.
    InterProiIPR004788. Ribose5P_isomerase_typA.
    IPR020672. Ribose5P_isomerase_typA_subgr.
    [Graphical view]
    PANTHERiPTHR11934. PTHR11934. 1 hit.
    PfamiPF06026. Rib_5-P_isom_A. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00021. rpiA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q72J47-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MERPLESYKK EAAHAAIAYV QDGMVVGLGT GSTARYAVLE LARRLREGEL
    60 70 80 90 100
    KGVVGVPTSR ATEELAKREG IPLVDLPPEG VDLAIDGADE IAPGLALIKG
    110 120 130 140 150
    MGGALLREKI VERAAKEFIV IADHTKKVPV LGRGPVPVEI VPFGYRATLK
    160 170 180 190 200
    AIADLGGEPE LRMDGDEFYF TDGGHLIADC RFGPIGDPLG LHRALLEIPG
    210 220
    VVETGLFVGM ATRALVAGPF GVEELLP
    Length:227
    Mass (Da):24,039
    Last modified:July 5, 2004 - v1
    Checksum:i47CD743AEAC126C7
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE017221 Genomic DNA. Translation: AAS81276.1.
    RefSeqiWP_011173356.1. NC_005835.1.

    Genome annotation databases

    EnsemblBacteriaiAAS81276; AAS81276; TT_C0932.
    KEGGitth:TTC0932.
    PATRICi23952267. VBITheThe54392_0926.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE017221 Genomic DNA. Translation: AAS81276.1.
    RefSeqiWP_011173356.1. NC_005835.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UJ4X-ray1.80A1-227[»]
    1UJ5X-ray2.00A1-227[»]
    1UJ6X-ray1.74A1-227[»]
    ProteinModelPortaliQ72J47.
    SMRiQ72J47. Positions 3-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi262724.TTC0932.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAS81276; AAS81276; TT_C0932.
    KEGGitth:TTC0932.
    PATRICi23952267. VBITheThe54392_0926.

    Phylogenomic databases

    eggNOGiENOG4105E66. Bacteria.
    COG0120. LUCA.
    KOiK01807.
    OMAiGACHVQE.
    OrthoDBiEOG67MF61.

    Enzyme and pathway databases

    UniPathwayiUPA00115; UER00412.
    BioCyciTTHE262724:GCAT-944-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiQ72J47.

    Family and domain databases

    HAMAPiMF_00170. Rib_5P_isom_A.
    InterProiIPR004788. Ribose5P_isomerase_typA.
    IPR020672. Ribose5P_isomerase_typA_subgr.
    [Graphical view]
    PANTHERiPTHR11934. PTHR11934. 1 hit.
    PfamiPF06026. Rib_5-P_isom_A. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00021. rpiA. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB27 / ATCC BAA-163 / DSM 7039.
    2. "Oxyanion hole-stabilized stereospecific isomerization in ribose-5-phosphate isomerase (Rpi)."
      Hamada K., Ago H., Sugahara M., Nodake Y., Kuramitsu S., Miyano M.
      J. Biol. Chem. 278:49183-49190(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS, FUNCTION, ACTIVE SITE, BIOPHYSICOCHEMICAL PROPERTIES, REACTION MECHANISM, SUBUNIT.

    Entry informationi

    Entry nameiRPIA_THET2
    AccessioniPrimary (citable) accession number: Q72J47
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: July 5, 2004
    Last modified: December 9, 2015
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.