Q72I16 (RL5_THET2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L5

Gene names

Name:	rplE
Ordered Locus Names:	TT_C1316

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs By similarity. HAMAP-Rule MF_01333
Subunit structure	Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L5P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 182

181

50S ribosomal protein L5 HAMAP-Rule MF_01333

PRO_0000125013

Secondary structure

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182

Helix Strand Turn

Details...

Helix

6 – 12

7

Helix

15 – 22

8

Turn

28 – 30

3

Beta strand

36 – 39

4

Turn

45 – 48

4

Helix

50 – 64

15

Turn

78 – 81

4

Beta strand

90 – 94

5

Helix

96 – 108

13

Helix

110 – 113

4

Helix

122 – 124

3

Beta strand

127 – 136

10

Helix

138 – 140

3

Beta strand

141 – 143

3

Turn

146 – 148

3

Beta strand

155 – 165

11

Helix

166 – 175

10

Sequences

	Sequence		Length	Mass (Da)
	Q72I16 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C3A8E0CFD884EEEA	FASTA	182	21,044
	10 20 30 40 50 60 MPLDLALKRK YYEEVRPELI RRFGYQNVWE VPRLEKVVIN QGLGEAKEDA RILEKAAQEL 70 80 90 100 110 120 ALITGQKPAV TRAKKSISNF KLRKGMPIGL RVTLRRDRMW IFLEKLLNVA LPRIRDFRGL 130 140 150 160 170 180 NPNSFDGRGN YNLGLREQLI FPEITYDMVD ALRGMDIAVV TTAETDEEAR ALLELLGFPF RK

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB27 / ATCC BAA-163 / DSM 7039.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE017221 Genomic DNA. Translation: AAS81658.1.

RefSeq

YP_005285.1. NC_005835.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VSA	X-ray	3.71	E	1-182	[»]
1VSP	X-ray	3.83	E	1-182	[»]
3D5B	X-ray	3.21	G	1-182	[»]
3D5D	X-ray	3.21	G	1-182	[»]
3F1F	X-ray	3.00	G	1-182	[»]
3F1H	X-ray	3.00	G	1-182	[»]
3MRZ	X-ray	3.62	F	2-182	[»]
3MS1	X-ray	3.62	F	2-182	[»]
3PYO	X-ray	3.50	F	2-182	[»]
3PYR	X-ray	3.50	F	2-182	[»]
3PYT	X-ray	3.40	F	2-182	[»]
3PYV	X-ray	3.40	F	2-182	[»]

ProteinModelPortal

Q72I16.

SMR

Q72I16. Positions 3-182.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAS81658; AAS81658; TT_C1316.

GeneID

2775564.

KEGG

tth:TTC1316.

PATRIC

23953039. VBITheThe54392_1308.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0094.

KO

K02931.

OMA

EQVMFHE.

ProtClustDB

PRK00010.

Family and domain databases

Gene3D

3.30.1440.10. 1 hit.

HAMAP

MF_01333_B. Ribosomal_L5_B.

InterPro

IPR002132. Ribosomal_L5.
IPR020930. Ribosomal_L5_bac-type.
IPR020929. Ribosomal_L5_CS.
IPR022803. Ribosomal_L5_domain.
[Graphical view]

PANTHER

PTHR11994. PTHR11994. 1 hit.

Pfam

PF00281. Ribosomal_L5. 1 hit.
PF00673. Ribosomal_L5_C. 1 hit.
[Graphical view]

PIRSF

PIRSF002161. Ribosomal_L5. 1 hit.

SUPFAM

SSF55282. Ribosomal_L5. 1 hit.

PROSITE

PS00358. RIBOSOMAL_L5. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q72I16.

Entry information

Entry name

RL5_THET2

Accession

Primary (citable) accession number: Q72I16

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program