Q72I07 (RL2_THET2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L2

Gene names

Name:	rplB
Ordered Locus Names:	TT_C1325

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity. HAMAP-Rule MF_01320
Subunit structure	Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 276

276

50S ribosomal protein L2 HAMAP-Rule MF_01320

PRO_0000237259

Secondary structure

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276

Helix Strand Turn

Details...

Beta strand

9 – 15

7

Beta strand

17 – 19

3

Helix

21 – 23

3

Beta strand

33 – 38

6

Beta strand

45 – 47

3

Beta strand

50 – 54

5

Beta strand

61 – 63

3

Turn

67 – 72

6

Beta strand

77 – 83

7

Beta strand

88 – 90

3

Beta strand

92 – 100

9

Beta strand

102 – 106

5

Beta strand

114 – 123

10

Beta strand

130 – 132

3

Turn

133 – 135

3

Beta strand

141 – 148

8

Turn

149 – 151

3

Beta strand

153 – 155

3

Beta strand

163 – 169

7

Beta strand

172 – 176

5

Beta strand

182 – 186

5

Beta strand

190 – 197

8

Helix

199 – 203

5

Helix

209 – 215

7

Helix

223 – 225

3

Turn

228 – 230

3

Turn

232 – 235

4

Beta strand

237 – 239

3

Beta strand

243 – 245

3

Helix

265 – 269

5

Sequences

	Sequence		Length	Mass (Da)
	Q72I07 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7CF40C2BD586349B	FASTA	276	30,468
	10 20 30 40 50 60 MAVKKFKPYT PSRRFMTVAD FSEITKTEPE KSLVKPLKKT GGRNNQGRIT VRFRGGGHKR 70 80 90 100 110 120 LYRIIDFKRW DKVGIPAKVA AIEYDPNRSA RIALLHYVDG EKRYIIAPDG LQVGQQVVAG 130 140 150 160 170 180 PDAPIQVGNA LPLRFIPVGT VVHAVELEPK KGAKLARAAG TSAQIQGREG DYVILRLPSG 190 200 210 220 230 240 ELRKVHGECY ATVGAVGNAD HKNIVLGKAG RSRWLGRRPH VRGAAMNPVD HPHGGGEGRA 250 260 270 PRGRPPASPW GWQTKGLKTR KRRKPSSRFI IARRKK

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB27 / ATCC BAA-163 / DSM 7039.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE017221 Genomic DNA. Translation: AAS81667.1.

RefSeq

YP_005294.1. NC_005835.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VSA	X-ray	3.71	B	1-276	[»]
1VSP	X-ray	3.83	B	1-276	[»]
3D5B	X-ray	3.21	D	1-276	[»]
3D5D	X-ray	3.21	D	1-276	[»]
3F1F	X-ray	3.00	D	1-276	[»]
3F1H	X-ray	3.00	D	1-276	[»]
3MRZ	X-ray	3.62	C	2-276	[»]
3MS1	X-ray	3.62	C	2-276	[»]
3PYO	X-ray	3.50	C	2-272	[»]
3PYR	X-ray	3.50	C	2-272	[»]
3PYT	X-ray	3.40	C	2-272	[»]
3PYV	X-ray	3.40	C	2-272	[»]

ProteinModelPortal

Q72I07.

SMR

Q72I07. Positions 2-273.

ModBase

Search...

Protein-protein interaction databases

STRING

262724.TTC1325.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAS81667; AAS81667; TT_C1325.

GeneID

2775711.

KEGG

tth:TTC1325.

PATRIC

23953057. VBITheThe54392_1317.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0090.

KO

K02886.

OMA

STIHCIE.

ProtClustDB

PRK09374.

Family and domain databases

Gene3D

2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.

HAMAP

MF_01320_B. Ribosomal_L2_B.

InterPro

IPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]

PANTHER

PTHR13691. PTHR13691. 1 hit.
PTHR13691:SF5. PTHR13691:SF5. 1 hit.

Pfam

PF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]

PIRSF

PIRSF002158. Ribosomal_L2. 1 hit.

SUPFAM

SSF50249. Nucleic_acid_OB. 1 hit.
SSF50104. Transl_SH3_like. 1 hit.

TIGRFAMs

TIGR01171. rplB_bact. 1 hit.

PROSITE

PS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q72I07.

Entry information

Entry name

RL2_THET2

Accession

Primary (citable) accession number: Q72I07

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program