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Protein

Elongation factor G

Gene

fusA

Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26GTPUniRule annotation8
Nucleotide bindingi83 – 87GTPUniRule annotation5
Nucleotide bindingi137 – 140GTPUniRule annotation4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor GUniRule annotation
Short name:
EF-GUniRule annotation
Gene namesi
Name:fusAUniRule annotation
Ordered Locus Names:TT_C1331
OrganismiThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Taxonomic identifieri262724 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000592 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000912471 – 691Elongation factor GAdd BLAST691

Interactioni

Protein-protein interaction databases

STRINGi262724.TTC1331.

Structurei

Secondary structure

1691
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 15Combined sources7
Beta strandi20 – 22Combined sources3
Helixi25 – 33Combined sources9
Turni56 – 61Combined sources6
Beta strandi66 – 68Combined sources3
Beta strandi73 – 76Combined sources4
Beta strandi77 – 82Combined sources6
Helixi93 – 98Combined sources6
Beta strandi104 – 107Combined sources4
Turni111 – 114Combined sources4
Helixi116 – 118Combined sources3
Helixi121 – 125Combined sources5
Beta strandi132 – 135Combined sources4
Beta strandi139 – 143Combined sources5
Helixi147 – 153Combined sources7
Turni154 – 157Combined sources4
Beta strandi165 – 168Combined sources4
Turni171 – 173Combined sources3
Beta strandi177 – 182Combined sources6
Beta strandi184 – 187Combined sources4
Beta strandi190 – 192Combined sources3
Beta strandi203 – 205Combined sources3
Helixi206 – 219Combined sources14
Turni220 – 223Combined sources4
Helixi227 – 232Combined sources6
Helixi239 – 251Combined sources13
Turni263 – 266Combined sources4
Helixi269 – 279Combined sources11
Turni283 – 285Combined sources3
Beta strandi290 – 292Combined sources3
Beta strandi294 – 296Combined sources3
Beta strandi298 – 300Combined sources3
Beta strandi310 – 317Combined sources8
Turni320 – 322Combined sources3
Beta strandi324 – 329Combined sources6
Beta strandi340 – 346Combined sources7
Beta strandi351 – 359Combined sources9
Beta strandi362 – 369Combined sources8
Beta strandi375 – 379Combined sources5
Beta strandi388 – 394Combined sources7
Beta strandi409 – 413Combined sources5
Turni416 – 420Combined sources5
Helixi421 – 434Combined sources14
Beta strandi439 – 442Combined sources4
Beta strandi444 – 456Combined sources13
Helixi457 – 469Combined sources13
Beta strandi484 – 486Combined sources3
Beta strandi491 – 495Combined sources5
Beta strandi509 – 514Combined sources6
Beta strandi530 – 534Combined sources5
Turni540 – 544Combined sources5
Helixi545 – 550Combined sources6
Turni554 – 556Combined sources3
Beta strandi569 – 571Combined sources3
Beta strandi574 – 576Combined sources3
Helixi579 – 595Combined sources17
Beta strandi601 – 608Combined sources8
Helixi615 – 626Combined sources12
Beta strandi627 – 629Combined sources3
Beta strandi638 – 642Combined sources5
Beta strandi645 – 647Combined sources3
Turni650 – 654Combined sources5
Helixi656 – 662Combined sources7
Beta strandi663 – 665Combined sources3
Beta strandi671 – 677Combined sources7
Helixi682 – 686Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V9JX-ray3.86AY/CY4-690[»]
4V9KX-ray3.50AY/CY4-690[»]
4V9LX-ray3.50AY/CY4-690[»]
4V9MX-ray4.00AY/CY4-690[»]
4W29X-ray3.80AY/CY4-690[»]
ProteinModelPortaliQ72I01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 284tr-type GAdd BLAST275

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CEJ. Bacteria.
COG0480. LUCA.
KOiK02355.
OMAiTEVQNYA.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q72I01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKVEYDLK RLRNIGIAAH IDAGKTTTTE RILYYTGRIH KIGEVHEGAA
60 70 80 90 100
TMDFMEQERE RGITITAAVT TCFWKDHRIN IIDTPGHVDF TIEVERSMRV
110 120 130 140 150
LDGAIVVFDS SQGVEPQSET VWRQAEKYHV PRIAFANKMD KTGADLWLVI
160 170 180 190 200
RTMQERLGAR PVVMQLPIGR EDTFSGIIDV LRMKAYTYGN DLGTDIREIP
210 220 230 240 250
IPEEYLDQAR EYHEKLVEVA ADFDEHIMLK YLEGEEPTEE ELVAAIRKGT
260 270 280 290 300
IDLKITPVFL GSALKNKGVQ LLLDAVVDYL PSPLDIPPIK GTTPEGEVVE
310 320 330 340 350
IHPDPNGPLA ALAFKIMADP YVGRLTFIRV YSGTLTSGSY VYNTTKGRKE
360 370 380 390 400
RVARLLRMHA NHREEVEELK AGDLGAVVGL KETITGDTLV GEDAPRVILE
410 420 430 440 450
SIEVPEPVID VAIEPKTKAD QEKLSQALAR LAEEDPTFRV STHPETGQTI
460 470 480 490 500
ISGMGELHLE IIVDRLKREF KVDANVGKPQ VAYRETITKP VDVEGKFIRQ
510 520 530 540 550
TGGRGQYGHV KIKVEPLPRG SGFEFVNAIV GGVIPKEYIP AVQKGIEEAM
560 570 580 590 600
QSGPLIGFPV VDIKVTLYDG SYHEVDSSEM AFKIAGSMAI KEAVQKGDPV
610 620 630 640 650
ILEPIMRVEV TTPEEYMGDV IGDLNARRGQ ILGMEPRGNA QVIRAFVPLA
660 670 680 690
EMFGYATDLR SKTQGRGSFV MFFDHYQEVP KQVQEKLIKG Q
Length:691
Mass (Da):76,911
Last modified:July 5, 2004 - v1
Checksum:i109D07619C9EEC81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017221 Genomic DNA. Translation: AAS81673.1.
RefSeqiWP_011173716.1. NC_005835.1.

Genome annotation databases

EnsemblBacteriaiAAS81673; AAS81673; TT_C1331.
KEGGitth:TT_C1331.
PATRICi23953069. VBITheThe54392_1323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017221 Genomic DNA. Translation: AAS81673.1.
RefSeqiWP_011173716.1. NC_005835.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V9JX-ray3.86AY/CY4-690[»]
4V9KX-ray3.50AY/CY4-690[»]
4V9LX-ray3.50AY/CY4-690[»]
4V9MX-ray4.00AY/CY4-690[»]
4W29X-ray3.80AY/CY4-690[»]
ProteinModelPortaliQ72I01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262724.TTC1331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS81673; AAS81673; TT_C1331.
KEGGitth:TT_C1331.
PATRICi23953069. VBITheThe54392_1323.

Phylogenomic databases

eggNOGiENOG4105CEJ. Bacteria.
COG0480. LUCA.
KOiK02355.
OMAiTEVQNYA.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFG_THET2
AccessioniPrimary (citable) accession number: Q72I01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.