Reviewed, UniProtKB/Swiss-Prot Q72GU1 (ODBA_THET2)

Names and origin

Protein names

Recommended name:
    2-oxoisovalerate dehydrogenase subunit alpha
    EC=1.2.4.4
Alternative name(s):
    Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
      Short name=BCKDH E1-alpha

Gene names

Ordered Locus Names:

TT_C1757

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO(2).
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Heterotetramer of two alpha and two beta chains. Directly associated with ODBB in the E1 complex By similarity.
Sequence similarities	Belongs to the BCKDHA family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	2-oxoisovalerate dehydrogenase subunit alpha		PRO_0000294974
Regions
	Region	94 – 96	3	Thiamine pyrophosphate binding By similarity
	Region	128 – 131	4	Substrate binding By similarity
	Region	144 – 146	3	Thiamine pyrophosphate binding By similarity
	Region	174 – 180	7	Thiamine pyrophosphate binding By similarity
	Region	204 – 208	5	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	175	1	Magnesium By similarity
	Metal binding	204	1	Magnesium By similarity
	Metal binding	206	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	273	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q72GU1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 726F33C315656E49	FASTA	367	41,442
	10 20 30 40 50 60 MVKETHRFEP FTEEPIRLIG EEGEWLGDFP LDLEGEKLRR LYRDMLAARM LDERYTILIR 70 80 90 100 110 120 TGKTSFIAPA AGHEAAQVAI AHAIRPGFDW VFPYYRDHGL ALALGIPLKE LFGQMLATKA 130 140 150 160 170 180 DPNKGRQMPE HPGSKALNFF TVASPIASHV PPAAGAAISM KLLRTGQVAV CTFGDGATSE 190 200 210 220 230 240 GDWYAGINFA AVQGAPAVFV CENNFYAISV DYRHQTHSPT IADKAHAFGI PGYLVDGMDV 250 260 270 280 290 300 LASYYVVKEA VERARRGEGP SLVELRVYRY GPHSSADDDS RYRPKEEVAF WRKKDPIPRF 310 320 330 340 350 360 RRFLEARGLW NEEWEEDVRE EIRAELERGL KEAEEAGPVP PEWMFADVFA EKPWHLLRQE ALLKEEL

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017221 Genomic DNA. Translation: AAS82099.1.
RefSeq	YP_005726.1.
3D structure databases
SMR	Q72GU1. Positions 6-367.
ModBase	Search...
Genome annotation databases
GeneID	2776443.
GenomeReviews	Gene locus TT_C1757 in contig AE017221_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q72GU1.
Enzyme and pathway databases
BioCyc	TTHE262724:TT_C1757-MON.
Family and domain databases
InterPro	IPR001017. DHase_E1. [Graphical view]
Pfam	PF00676. E1_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ODBA_THET2

Accession

Primary (citable) accession number: Q72GU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 5, 2004
Last modified:	November 4, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)