ID   Q72GJ6_THET2            Unreviewed;       378 AA.
AC   Q72GJ6;
DT   05-JUL-2004, integrated into UniProtKB/TrEMBL.
DT   05-JUL-2004, sequence version 1.
DT   27-MAR-2024, entry version 119.
DE   RecName: Full=Peptide chain release factor 2 {ECO:0000256|HAMAP-Rule:MF_00094};
DE            Short=RF-2 {ECO:0000256|HAMAP-Rule:MF_00094};
GN   Name=prfB {ECO:0000256|HAMAP-Rule:MF_00094};
GN   OrderedLocusNames=TT_C1852 {ECO:0000313|EMBL:AAS82194.1};
OS   Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27).
OC   Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=262724 {ECO:0000313|EMBL:AAS82194.1, ECO:0000313|Proteomes:UP000000592};
RN   [1] {ECO:0000313|EMBL:AAS82194.1, ECO:0000313|Proteomes:UP000000592}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-163 / DSM 7039 / HB27
RC   {ECO:0000313|Proteomes:UP000000592};
RX   PubMed=15064768; DOI=10.1038/nbt956;
RA   Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H.,
RA   Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C.,
RA   Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P.,
RA   Overbeek R., Kramer W., Merkl R., Gottschalk G., Fritz H.-J.;
RT   "The genome sequence of the extreme thermophile Thermus thermophilus.";
RL   Nat. Biotechnol. 22:547-553(2004).
RN   [2] {ECO:0007829|PDB:4V9N}
RP   X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS) OF 17-378.
RX   PubMed=23769667; DOI=10.1016/j.str.2013.04.028;
RA   Santos N., Zhu J., Donohue J.P., Korostelev A.A., Noller H.F.;
RT   "Crystal structure of the 70S ribosome bound with the Q253P mutant form of
RT   release factor RF2.";
RL   Structure 21:1258-1263(2013).
CC   -!- FUNCTION: Peptide chain release factor 2 directs the termination of
CC       translation in response to the peptide chain termination codons UGA and
CC       UAA. {ECO:0000256|HAMAP-Rule:MF_00094}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00094}.
CC   -!- PTM: Methylated by PrmC. Methylation increases the termination
CC       efficiency of RF2. {ECO:0000256|HAMAP-Rule:MF_00094}.
CC   -!- SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor
CC       family. {ECO:0000256|ARBA:ARBA00010835, ECO:0000256|HAMAP-
CC       Rule:MF_00094}.
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DR   EMBL; AE017221; AAS82194.1; -; Genomic_DNA.
DR   PDB; 4V9N; X-ray; 3.40 A; AY/CY=17-378.
DR   PDBsum; 4V67; -.
DR   PDBsum; 4V9N; -.
DR   AlphaFoldDB; Q72GJ6; -.
DR   SMR; Q72GJ6; -.
DR   IntAct; Q72GJ6; 52.
DR   KEGG; tth:TT_C1852; -.
DR   eggNOG; COG1186; Bacteria.
DR   HOGENOM; CLU_036856_6_0_0; -.
DR   Proteomes; UP000000592; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016149; F:translation release factor activity, codon specific; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.160.20; -; 1.
DR   Gene3D; 3.30.70.1660; -; 1.
DR   Gene3D; 1.20.58.410; Release factor; 1.
DR   HAMAP; MF_00094; Rel_fac_2; 1.
DR   InterPro; IPR005139; PCRF.
DR   InterPro; IPR000352; Pep_chain_release_fac_I.
DR   InterPro; IPR045853; Pep_chain_release_fac_I_sf.
DR   InterPro; IPR004374; PrfB.
DR   NCBIfam; TIGR00020; prfB; 1.
DR   PANTHER; PTHR43116; PEPTIDE CHAIN RELEASE FACTOR 2; 1.
DR   PANTHER; PTHR43116:SF3; RF_PROK_I DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF03462; PCRF; 1.
DR   Pfam; PF00472; RF-1; 1.
DR   SMART; SM00937; PCRF; 1.
DR   SUPFAM; SSF75620; Release factor; 1.
DR   PROSITE; PS00745; RF_PROK_I; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4V9N}; Coiled coil {ECO:0000256|SAM:Coils};
KW   Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00094};
KW   Methylation {ECO:0000256|ARBA:ARBA00022481, ECO:0000256|HAMAP-
KW   Rule:MF_00094}; Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00094}.
FT   DOMAIN          246..262
FT                   /note="Prokaryotic-type class I peptide chain release
FT                   factors"
FT                   /evidence="ECO:0000259|PROSITE:PS00745"
FT   COILED          282..314
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   MOD_RES         253
FT                   /note="N5-methylglutamine"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00094"
SQ   SEQUENCE   378 AA;  42734 MW;  55B4AC9C891AEEB7 CRC64;
     MRLASQSAIL VKVWTWNASR NAWKASGGIF DIPQKETRLK ELERRLEDPS LWNDPEAARK
     VSQEAARLRR TVDTFRSLES DLQGLLELME ELPAEEREAL KPELEEAAKK LDELYHQTLL
     NFPHAEKNAI LTIQPGAGGT EACDWAEMLL RMYTRFAERQ GFQVEVVDLT PGPEAGIDYA
     QILVKGENAY GLLSPEAGVH RLVRPSPFDA SGRRHTSFAG VEVIPEVDEE VEVVLKPEEL
     RIDVMRASGP GGQGVNTTDS AVRVVHLPTG ITVTCQTTRS QIKNKELALK ILKARLYELE
     RKKREEELKA LRGEVRPIEW GSQIRSYVLD KNYVKDHRTG LMRHDPENVL DGDLMDLIWA
     GLEWKAGRRQ GTEEVEAE
//