Reviewed, UniProtKB/Swiss-Prot Q72GD6 (NQO9_THET2)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 9
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit 9
    NDH-1 subunit 9

Gene names

Name:	nqo9
Ordered Locus Names:	TT_C1912

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The role of the nqo9 subunit appears to provide a 'connecting chain' of two clusters between cluster N5 and the terminal cluster N2, and to stabilize the structure of the complex by interacting with other subunits By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01351
Cofactor	Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	NADH-quinone oxidoreductase subunit 9 HAMAP MF_01351		PRO_0000245755
Regions
	Domain	43 – 73	31	4Fe-4S ferredoxin-type 1
	Domain	89 – 118	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	53	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	56	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	63	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	98	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	101	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	104	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	108	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q72GD6-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 054A9F7942C64C66	FASTA	182	20,080
	10 20 30 40 50 60 MTLKALAQSL GITLKYLFSK PVTVPYPDAP VALKPRFHGR HVLTRHPNGL EKCIGCSLCA 70 80 90 100 110 120 AACPAYAIYV EPAENDPENP VSAGERYAKV YEINMLRCIF CGLCEEACPT GAIVLGYDFE 130 140 150 160 170 180 MADYEYSDLV YGKEDMLVDV VGTKPQRREA KRTGKPVKVG YVVPYVRPEL EGFKAPTEGG KR

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017221 Genomic DNA. Translation: AAS82254.1.
RefSeq	YP_005881.1.
3D structure databases
SMR	Q72GD6. Positions 26-179.
ModBase	Search...
Protein-protein interaction databases
STRING	Q72GD6.
Genome annotation databases
GeneID	2775293.
GenomeReviews	Gene locus TT_C1912 in contig AE017221_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q72GD6.
OMA	FARCIFC.
Enzyme and pathway databases
BioCyc	TTHE262724:TT_C1912-MON.
Family and domain databases
HAMAP	MF_01351. [Tree]
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. [Graphical view]
TIGRFAMs	TIGR01971. NuoI. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO9_THET2

Accession

Primary (citable) accession number: Q72GD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)