Reviewed, UniProtKB/Swiss-Prot Q72GD5 (NQO8_THET2)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 8
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit 8
    NDH-1 subunit 8

Gene names

Name:	nqo8
Ordered Locus Names:	TT_C1913

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP By similarity.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane proteinPotential.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	NADH-quinone oxidoreductase subunit 8		PRO_0000240113
Regions
	Transmembrane	11 – 31	21	Potential
	Transmembrane	80 – 100	21	Potential
	Transmembrane	120 – 140	21	Potential
	Transmembrane	157 – 177	21	Potential
	Transmembrane	192 – 212	21	Potential
	Transmembrane	252 – 272	21	Potential
	Transmembrane	273 – 293	21	Potential
	Transmembrane	310 – 330	21	Potential
	Transmembrane	336 – 356	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q72GD5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5B920A702F858C0F	FASTA	365	41,009
	10 20 30 40 50 60 MTWSYPVDPY WMVALKALLV VVGLLTAFAF MTLIERRLLA RFQVRMGPNR VGPFGLLQPL 70 80 90 100 110 120 ADAIKSIFKE DIVVAQADRF LFVLAPLISV VFALLAFGLI PFGPPGSFFG YQPWVINLDL 130 140 150 160 170 180 GILYLFAVSE LAVYGIFLSG WASGSKYSLL GSLRSSASLI SYELGLGLAL LAPVLLVGSL 190 200 210 220 230 240 NLNDIVNWQK EHGWLFLYAF PAFLVYLIAS MAEAARTPFD LPEAEQELVG GYHTEYSSIK 250 260 270 280 290 300 WALFQMAEYI HFITASALIP TLFLGGWTMP VLEVPYLWMF LKLAFFLFFF IWIRATWFRL 310 320 330 340 350 360 RYDQLLRFGW GFLFPLALLW FLVTALVVAL DLPRTYLLYL SALSFLVLLG AVLYTPKPAR KGGGA

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017221 Genomic DNA. Translation: AAS82255.1.
RefSeq	YP_005882.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2775646.
GenomeReviews	Gene locus TT_C1913 in contig AE017221_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q72GD5.
Enzyme and pathway databases
BioCyc	TTHE262724:TT_C1913-MON.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. Resp_NADH_DHase_1. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO8_THET2

Accession

Primary (citable) accession number: Q72GD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	July 5, 2004
Last modified:	November 4, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)