Reviewed, UniProtKB/Swiss-Prot Q72GD0 (NQO5_THET2)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 5
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I chain 5
    NDH-1 subunit 5

Gene names

Name:	nqo5
Ordered Locus Names:	TT_C1918

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo5 subunit may be involved in the stabilization of the complex By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01357
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. The N-terminal domain of subunit nqo5 (residues 1 to 153) wraps around nqo4 on one side, and the subunit interacts also with nqo9 via a two-stranded beta sheet (residues 154 to 171) and with nqo3 via an extended C-terminal loop (residues 172 to 196) By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	NADH-quinone oxidoreductase subunit 5 HAMAP MF_01357		PRO_0000358215

Sequences

	Sequence		Length	Mass (Da)
	Q72GD0-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EB6891BEF6F314AD	FASTA	207	23,859
	10 20 30 40 50 60 MRLERVLEEA RAKGYPIEDN GLGNLWVVLP RERFKEEMAH YKAMGFNFLA DIVGLDYLTY 70 80 90 100 110 120 PDPRPERFAV VYELVSLPGW KDGDGSRFFV RVYVPEEDPR LPTVTDLWGS ANFLEREVYD 130 140 150 160 170 180 LFGIVFEGHP DLRKILTPED LEGHPLRKDY PLGETPTLFR EGRYIIPAEF RAALTGKDPG 190 200 LTFYKGGSRK GYRSLWADLK KAREVKG

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017221 Genomic DNA. Translation: AAS82260.1.
RefSeq	YP_005887.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q72GD0.
Genome annotation databases
GeneID	2775512.
GenomeReviews	Gene locus TT_C1918 in contig AE017221_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q72GD0.
OMA	ERECHEM.
Enzyme and pathway databases
BioCyc	TTHE262724:TT_C1918-MON.
Family and domain databases
HAMAP	MF_01357. [Tree]
InterPro	IPR010218. NADH_DH_csu. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR020396. NADH_UbQ_OxRdtase_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO5_THET2

Accession

Primary (citable) accession number: Q72GD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)