Q72GB7 (Q72GB7_THET2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

TT_C1931

Organism

Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) [Complete proteome] [HAMAP]

Taxonomic identifier

262724 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	824 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q72GB7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 13D8DDED56A3D9F3	FASTA	824	92,789
	10 20 30 40 50 60 MPKSAKRRAS QPTGAETLVV VESPAKARSI QKMLGPGYEV RASLGHVVDL PERSLGVDVE 70 80 90 100 110 120 RDFAPTYEVK KDKKPVVEEL KRAAEGKRLL IATDPDREGE AIGWHVARLL GRDPKEPLRV 130 140 150 160 170 180 EFHEITPRVV RQAVEAPRPI DQNLVDAQQA RRVLDRLLGY NLSPLLSLEF RKRALSAGRV 190 200 210 220 230 240 QSVALRLVVE REEAIEAFRP EEYWVLRGVF AVEGGEAPLV ATLHEVEGKR LWTGQGEKEG 250 260 270 280 290 300 RLHLASEAEA HALAEALRKG SYRVAQVEVR ERRKAPPPPF TTSTLQQAAS SRLGYTAART 310 320 330 340 350 360 MRIAQRLYEG VDLPEGTVGL ITYMRTDSVR VAPEAVAAAR EVIGKAFGPE YLPEAPRAYK 370 380 390 400 410 420 NRREGVQDAH EAIRPTDPQR TPESVRPYLS EEEYRLYELI WRRFLASQMR DALYEQTAVT 430 440 450 460 470 480 LADLEGRYAF RASGSVLRFE GYLRAWGRDA EEEEEPPVPA VPEGVPARLE EVLEERRFTE 490 500 510 520 530 540 PPPRYTDASL VKTMEELGIG RPSTYAPTLE TLEKRGYVAR KGRTLFPTPL GRQVTAYLRE 550 560 570 580 590 600 RFPQVVAYEF TARMEERLDE VEEGRVPWPK VVWEFYEPFL GELAQVPKKT CPECGRPLEL 610 620 630 640 650 660 KVSRYGQFLG CTGYPECTYT EPLERREAEP IGEACPKCGR PLLRKEGRYG AFIACSGYPE 670 680 690 700 710 720 CDYTRDDGTP TGHTCPKCGG RVLEKRSRKG RPYYKCENNA CDFLSFYPLL EEACPACGWP 730 740 750 760 770 780 LVKKGEKALC LNPACPEHEP SLLPAPKEAK KGRSRGKGKP PLAPPKDWEE LVPFLPGLSP 790 800 810 820 EERRAVEALA RGEALAEEEA RLARRALFKL RMRKGKARKE PVRA

References

[1]

"The genome sequence of the extreme thermophile Thermus thermophilus." Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H., Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C., Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P., Kramer W., Merkl R., Gottschalk G., Fritz H.-J. Nat. Biotechnol. 22:547-553(2004) [PubMed: 15064768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017221 Genomic DNA. Translation: AAS82273.1.
RefSeq	YP_005900.1. NC_005835.1.
3D structure databases
ProteinModelPortal	Q72GB7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q72GB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2776208.
GenomeReviews	Gene locus TT_C1931 in contig AE017221_GR.
PATRIC	23954335. VBITheThe54392_1927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	Q72GB7.
ProtClustDB	PRK06599.
Enzyme and pathway databases
BioCyc	TTHE262724:TT_C1931-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q72GB7_THET2

Accession

Primary (citable) accession number: Q72GB7

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)