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Q72AR5

- SYI_DESVH

UniProt

Q72AR5 - SYI_DESVH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei566 – 5661Aminoacyl-adenylateUniRule annotation
    Binding sitei610 – 6101ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDVUL882:GJIL-1970-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:DVU_1927
    OrganismiDesulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303)
    Taxonomic identifieri882 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio
    ProteomesiUP000002194: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_0000098380Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi882.DVU1927.

    Structurei

    3D structure databases

    ProteinModelPortaliQ72AR5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi607 – 6115"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.
    PhylomeDBiQ72AR5.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q72AR5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKKTLQL PETKFPMKAN LTQREPEMLR KWEKDDAYGA MVRASGQQGT    50
    YVLHDGPPYA NGNIHMGHAL NKILKDIIVK SRNLQGFKAE YVPGWDCHGL 100
    PIELKVEHEL GEKKRTMPAH AVRKRCRQYA EKYLDIQRKE FKRLGVFGAW 150
    DKPYVTMHPS YEAATARELG NFAAKGGLVR SKKPIYWCCS CQTALAEAEV 200
    EYHDHTSPSV HVRFPLRDPR VAEVLPGVDP AHAYIVIWTT TPWTLPDNMA 250
    VAVHPDFDYV VVRHGGDFHI VAEGLLEACL KAFKWDEHEV VARIGGRALE 300
    GLKATHPFYD RPSPIVLADY VTLESGTGCV HTAPGHGRED YETGLRYGLD 350
    IYSPLTDEGR YLDCVEFFAG MTIFEANPKV IEKLREVGNL LAEGRITHSY 400
    PHCWRCKKPV IFRATTQWFI AMERNDLRQK ALDAIRDDVR WIPSWGQERI 450
    HNMIEFRPDW CISRQRMWGV PIVALLCEDC GEAWNDADWM RDIAERFAKH 500
    ATGCDYWYET DLSDIVPAGL RCPKCGGDHW KKETDILDVW FDSGTSFAAV 550
    VEQREECGFP ADLYLEGSDQ HRGWFHSSLL ASIGTRGVPP YRSVLTHGYV 600
    VDGDGRKMSK SVGNVVAPQE IIDKHGAEVL RLWVASVDYR EDIRISEEIL 650
    NRLVDAYRRI RNTCRYLLGN ISDLTPETMV PFEAMDPLDR FALDLASRAH 700
    ERIQDAYTEY EFHKVFHTLH NLCVTDLSAF YLDILKDRLY SSAADSHARR 750
    SAQTALYRIL MLMVRDMAPV LSFTAEEVFG YVPAALRPDV ISVFALPATD 800
    APAFTLDTTS RAAWEKLLAV RSETTKAIEP LRKSGEVGHS LDTHVTLFAD 850
    PSLKATLEGL GSDLRAMFIV SRLEVMDLAD APADAWTSEE LPELKVTVRK 900
    AEGEKCERCW IISADLGTDA AHPTLCPRCT AVLTGTGA 938
    Length:938
    Mass (Da):105,978
    Last modified:July 5, 2004 - v1
    Checksum:i45ACF848190669FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017285 Genomic DNA. Translation: AAS96403.1.
    RefSeqiYP_011144.1. NC_002937.3.

    Genome annotation databases

    EnsemblBacteriaiAAS96403; AAS96403; DVU_1927.
    GeneIDi2795304.
    KEGGidvu:DVU1927.
    PATRICi32063594. VBIDesVul119526_1770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017285 Genomic DNA. Translation: AAS96403.1 .
    RefSeqi YP_011144.1. NC_002937.3.

    3D structure databases

    ProteinModelPortali Q72AR5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 882.DVU1927.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS96403 ; AAS96403 ; DVU_1927 .
    GeneIDi 2795304.
    KEGGi dvu:DVU1927.
    PATRICi 32063594. VBIDesVul119526_1770.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.
    PhylomeDBi Q72AR5.

    Enzyme and pathway databases

    BioCyci DVUL882:GJIL-1970-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hildenborough / ATCC 29579 / NCIMB 8303.

    Entry informationi

    Entry nameiSYI_DESVH
    AccessioniPrimary (citable) accession number: Q72AR5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3