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Q728P5 (BIOB_DESVH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:DVU_2558
OrganismDesulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) [Reference proteome] [HAMAP]
Taxonomic identifier882 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfovibrionaceaeDesulfovibrio

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence AAS97030.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362Biotin synthase HAMAP-Rule MF_01694
PRO_0000381351

Sites

Metal binding1051Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1091Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1121Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1811Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2411Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q728P5 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 10BE195AF0ECF63B

FASTA36238,731
        10         20         30         40         50         60 
MHNLLENLRR RLLAQRTPEP LPPTSQGLAR PSHDVVRGPC DADIPPDARN TMMPEGITVE 

        70         80         90        100        110        120 
EALAVAELPQ KHALDILATA QAIRSVHKGG PAALCGIVNA KSGRCPEDCA FCAQSSHHAT 

       130        140        150        160        170        180 
GSPVHALLDA ETLLRRAEEL RQSGAERYGI VTSGTRLTVR ELATLCEAAV RIRRETGIAL 

       190        200        210        220        230        240 
CGSLGQLTPD AAACLKEAGF SSYHHNLETS RSFFPAICST HAYDDDIATV RAARAAGLRT 

       250        260        270        280        290        300 
CSGGIFGMGE TDAQRIELSA TLRELDVDSI PVNLLSPIPG TPLQHRPTMP PMRALVSIAI 

       310        320        330        340        350        360 
YRLMHPARDI LVCGGREATL GPWQSWIFLA GANGMMVGNY LTTTGRDMAD DLAMLATLGV 


RA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017285 Genomic DNA. Translation: AAS97030.1. Different initiation.
RefSeqYP_011770.1. NC_002937.3.

3D structure databases

ProteinModelPortalQ728P5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING882.DVU2558.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS97030; AAS97030; DVU_2558.
GeneID2796132.
KEGGdvu:DVU2558.
PATRIC32064720. VBIDesVul119526_2316.

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.
ProtClustDBCLSK705864.

Enzyme and pathway databases

BioCycDVUL882:GJIL-2620-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_DESVH
AccessionPrimary (citable) accession number: Q728P5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways