Q725C8 (PTC_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putrescine carbamoyltransferase
Short name=PTC
Short name=PTCase
EC=2.1.3.6

Alternative name(s):
Putrescine transcarbamoylase
Putrescine transcarbamylase

Gene names

Name:	ptcA
Synonyms:	argF-1
Ordered Locus Names:	LMOf2365_0045

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine By similarity. HAMAP MF_02102
Catalytic activity	Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine. HAMAP MF_02102
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from N-carbamoylputrescine (transferase route): step 1/1. HAMAP MF_02102
Subunit structure	Homotrimer By similarity. HAMAP MF_02102
Subcellular location	Cytoplasm Probable HAMAP MF_02102.
Sequence similarities	Belongs to the ATCase/OTCase family. PTCase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	polyamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: InterPro putrescine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Putrescine carbamoyltransferase HAMAP MF_02102		PRO_0000112944
Regions
	Region	54 – 58	5	Carbamoyl phosphate binding By similarity
	Region	271 – 274	4	Putrescine binding By similarity
Sites
	Binding site	105	1	Carbamoyl phosphate By similarity
	Binding site	132	1	Carbamoyl phosphate By similarity
	Site	29	1	Important for structural integrity By similarity
	Site	145	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q725C8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8027DF93BDBE9D12	FASTA	341	38,469
	10 20 30 40 50 60 MNKKRDFIDT KDFSKEEILF MIEIGRKMKE SIKNGHYPQL LKHKTLGMIF EQSSTRTRVS 70 80 90 100 110 120 FETAMTQLGG HAQYLAPGQI QLGGHESVGD TAKVLSRLVD ILMARVERHQ TVVELANTAA 130 140 150 160 170 180 IPVINGMSDY NHPTQELGDA ITMFEHLPKG KKIEDCKIVF VGDATQVCAS TMFMATKLGM 190 200 210 220 230 240 DFVQFGPKGF QLREEHLKIA EENCKVSGGS YLITEDVDIA LKDADFIYTD VWYGLYEAEL 250 260 270 280 290 300 SEEERMKTFY PKYQVNKELI SKAAPHVKFM HCLPATRGEE VTDEVLDAPY SVVIDEAENR 310 320 330 340 LTAMRALLVY FMNPYVREAG FAVAEKYDAE LELLLRNGAG L

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT02833.1.
RefSeq	YP_012656.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q725C8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q725C8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2799360.
GenomeReviews	Gene locus LMOf2365_0045 in contig AE017262_GR.
KEGG	lmf:LMOf2365_0045.
PATRIC	20321262. VBILisMon105049_0046.
TIGR	LMOf2365_0045.
Phylogenomic databases
eggNOG	COG0078.
HOGENOM	HBG579429.
OMA	LGMIFQQ.
PhylomeDB	Q725C8.
ProtClustDB	PRK02255.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_0045-MONOMER.
Family and domain databases
HAMAP	MF_02102. PTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024903. PtcA. [Graphical view]
KO	K13252.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTC_LISMF

Accession

Primary (citable) accession number: Q725C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program