Reviewed, UniProtKB/Swiss-Prot Q724L4 (KPRS1_LISMF)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 1
      Short name=RPPK 1
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 1
      Short name=P-Rib-PP synthetase 1
      Short name=PRPP synthetase 1

Gene names

Name:	prs1
Ordered Locus Names:	LMOf2365_0210

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity HAMAP MF_00583.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Ribose-phosphate pyrophosphokinase 1 HAMAP MF_00583		PRO_0000141155
Regions
	Region	218 – 231	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	136	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential
	Metal binding	149	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q724L4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: AB286F742362FECD	FASTA	318	35,069
	10 20 30 40 50 60 MSNEYFDPKL KIFSLNSNRE LAEEIAKEVG IELGKSSVTH FSDGEIQINI EESIRGCHVY 70 80 90 100 110 120 VIQSTSNPVN QNLMELLIMI DALKRASAAT INIVMPYYGY ARQDRKARSR EPITAKLVAN 130 140 150 160 170 180 LIETAGATRM ITLDMHAPQI QGFFDIPIDH LNAVRLLSDY FSERHLGDDL VVVSPDHGGV 190 200 210 220 230 240 TRARKMADRL KAPIAIIDKR RPRPNVAEVM NIVGNVEGKV CIIIDDIIDT AGTITLAAKA 250 260 270 280 290 300 LREAGATKVY ACCSHPVLSG PAMKRIEESP IEKLVVTNSI ALPEEKWIDK MEQLSVAALL 310 GEAIVRVHEN ASVSSLFE

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT02997.1.
RefSeq	YP_012820.1.
3D structure databases
SMR	Q724L4. Positions 2-317.
ModBase	Search...
Protein-protein interaction databases
STRING	Q724L4.
Genome annotation databases
GeneID	2797245.
GenomeReviews	Gene locus LMOf2365_0210 in contig AE017262_GR.
KEGG	lmf:LMOf2365_0210.
TIGR	LMOf2365_0210.
Phylogenomic databases
eggNOG	COG0462.
HOGENOM	HBG519284.
OMA	CATHAVF.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_0210-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_LISMF

Accession

Primary (citable) accession number: Q724L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)