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Q724J4

- TILS_LISMF

UniProt

Q724J4 - TILS_LISMF

Protein

Bifunctional protein TilS/HprT

Gene

tilS/hprT

Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.
    (tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.

    Cofactori

    Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi627 – 6271Magnesium 1By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 346ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    3. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
    4. ligase activity, forming carbon-nitrogen bonds Source: UniProtKB-HAMAP
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. purine ribonucleoside salvage Source: InterPro
    2. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Ligase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein TilS/HprT
    Including the following 2 domains:
    tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
    Alternative name(s):
    tRNA(Ile)-2-lysyl-cytidine synthase
    tRNA(Ile)-lysidine synthetase
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:tilS/hprT
    Ordered Locus Names:LMOf2365_0230
    OrganismiListeria monocytogenes serotype 4b (strain F2365)
    Taxonomic identifieri265669 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000000563: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 648648Bifunctional protein TilS/HprTPRO_0000181717Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi265669.LMOf2365_0230.

    Structurei

    3D structure databases

    ProteinModelPortaliQ724J4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

    Sequence similaritiesi

    In the N-terminal section; belongs to the tRNA(Ile)-lysidine synthase family.Curated
    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0634.
    HOGENOMiHOG000217053.
    KOiK15780.
    OMAiNDISYPL.
    OrthoDBiEOG693GNP.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_01161. tRNA_Ile_lys_synt.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR012094. Lysidine-tRNA-synth.
    IPR012796. Lysidine-tRNA-synth_C.
    IPR012795. Lysidine-tRNA-synth_N.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR011063. tRNA-lysidine/thiocyt_synth.
    [Graphical view]
    PfamiPF01171. ATP_bind_3. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    PF11734. TilS_C. 1 hit.
    [Graphical view]
    SMARTiSM00977. TilS_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    TIGR02433. lysidine_TilS_C. 1 hit.
    TIGR02432. lysidine_TilS_N. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q724J4-1 [UniParc]FASTAAdd to Basket

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    MDDTDKRVHK YIEKHDLIRS DDKLLVAVSG GPDSLALLHF LWNSNLVPKE    50
    AISVAHLNHH LRENAANEQN VVETFCESQG IPFYIEEVDV KSRAESLQKG 100
    IEETARIVRY DFFEKVMAEK KINKLALAHH ADDQIETILM RLVRGSASIG 150
    WSGIQPKREL KGGQAIRPFL PITKAEIIDY AQKHELAYEI DESNTSQEYT 200
    RNRYRAQLLP FLKQENPAVY SHFERFSEET SEDFQFLEAL ASDLLKKNLI 250
    KNGKRTTLLL TNFKNEANPL QRRAIHLLLR YLYNEDASFI TVNHIYQIIQ 300
    MIQSDNPSSS IDLPNKLVAN RAYDKLHFQF GEREAPSEFY HQLELNDRIE 350
    LDNKASIRLK LKSSVVQTNG LNGMLLDAEE ITLPLIVRNR VNGDRMTMKG 400
    QAGSKKLKDI FIDAKIPRQE RDKLPVITDY TGKILWVPGV KKSAYDREFS 450
    RSKKQYIIRY TRNIGGNESM HNDIQKVLIS EDELQEKIRE LGRELTTEYE 500
    GRNPLVVGVL KGATPFMTDL LKRVDTYLEM DFMDVSSYGN GTVSSGEVKI 550
    IKDLNASVEG RDVLVIEDII DSGRTLSYLV DLIKYRKAKS VKLVTLLDKP 600
    AGRNVEIEAD YVGFVVPNEF VVGYGLDYAE RYRNLPYIGI LKPEIYSE 648
    Length:648
    Mass (Da):74,266
    Last modified:July 5, 2004 - v1
    Checksum:iE181EE42A0D39129
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017262 Genomic DNA. Translation: AAT03017.1.
    RefSeqiYP_012840.1. NC_002973.6.

    Genome annotation databases

    EnsemblBacteriaiAAT03017; AAT03017; LMOf2365_0230.
    GeneIDi2797333.
    KEGGilmf:LMOf2365_0230.
    PATRICi20321639. VBILisMon105049_0233.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017262 Genomic DNA. Translation: AAT03017.1 .
    RefSeqi YP_012840.1. NC_002973.6.

    3D structure databases

    ProteinModelPortali Q724J4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 265669.LMOf2365_0230.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT03017 ; AAT03017 ; LMOf2365_0230 .
    GeneIDi 2797333.
    KEGGi lmf:LMOf2365_0230.
    PATRICi 20321639. VBILisMon105049_0233.

    Phylogenomic databases

    eggNOGi COG0634.
    HOGENOMi HOG000217053.
    KOi K15780.
    OMAi NDISYPL.
    OrthoDBi EOG693GNP.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_01161. tRNA_Ile_lys_synt.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR012094. Lysidine-tRNA-synth.
    IPR012796. Lysidine-tRNA-synth_C.
    IPR012795. Lysidine-tRNA-synth_N.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR011063. tRNA-lysidine/thiocyt_synth.
    [Graphical view ]
    Pfami PF01171. ATP_bind_3. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    PF11734. TilS_C. 1 hit.
    [Graphical view ]
    SMARTi SM00977. TilS_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    TIGR02433. lysidine_TilS_C. 1 hit.
    TIGR02432. lysidine_TilS_N. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: F2365.

    Entry informationi

    Entry nameiTILS_LISMF
    AccessioniPrimary (citable) accession number: Q724J4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3