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Q724J4

- TILS_LISMF

UniProt

Q724J4 - TILS_LISMF

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Protein

Bifunctional protein TilS/HprT

Gene
tilS/hprT, LMOf2365_0230
Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity.UniRule annotation

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Cofactori

Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi627 – 6271Magnesium 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 346ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  4. ligase activity, forming carbon-nitrogen bonds Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. purine ribonucleoside salvage Source: InterPro
  2. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Ligase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein TilS/HprT
Including the following 2 domains:
tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:tilS/hprT
Ordered Locus Names:LMOf2365_0230
OrganismiListeria monocytogenes serotype 4b (strain F2365)
Taxonomic identifieri265669 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000563: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648Bifunctional protein TilS/HprTUniRule annotationPRO_0000181717Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi265669.LMOf2365_0230.

Structurei

3D structure databases

ProteinModelPortaliQ724J4.

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the tRNA(Ile)-lysidine synthase family.
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000217053.
KOiK15780.
OMAiNDISYPL.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR005904. Hxn_phspho_trans.
IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PfamiPF01171. ATP_bind_3. 1 hit.
PF00156. Pribosyltran. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
TIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q724J4-1 [UniParc]FASTAAdd to Basket

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MDDTDKRVHK YIEKHDLIRS DDKLLVAVSG GPDSLALLHF LWNSNLVPKE    50
AISVAHLNHH LRENAANEQN VVETFCESQG IPFYIEEVDV KSRAESLQKG 100
IEETARIVRY DFFEKVMAEK KINKLALAHH ADDQIETILM RLVRGSASIG 150
WSGIQPKREL KGGQAIRPFL PITKAEIIDY AQKHELAYEI DESNTSQEYT 200
RNRYRAQLLP FLKQENPAVY SHFERFSEET SEDFQFLEAL ASDLLKKNLI 250
KNGKRTTLLL TNFKNEANPL QRRAIHLLLR YLYNEDASFI TVNHIYQIIQ 300
MIQSDNPSSS IDLPNKLVAN RAYDKLHFQF GEREAPSEFY HQLELNDRIE 350
LDNKASIRLK LKSSVVQTNG LNGMLLDAEE ITLPLIVRNR VNGDRMTMKG 400
QAGSKKLKDI FIDAKIPRQE RDKLPVITDY TGKILWVPGV KKSAYDREFS 450
RSKKQYIIRY TRNIGGNESM HNDIQKVLIS EDELQEKIRE LGRELTTEYE 500
GRNPLVVGVL KGATPFMTDL LKRVDTYLEM DFMDVSSYGN GTVSSGEVKI 550
IKDLNASVEG RDVLVIEDII DSGRTLSYLV DLIKYRKAKS VKLVTLLDKP 600
AGRNVEIEAD YVGFVVPNEF VVGYGLDYAE RYRNLPYIGI LKPEIYSE 648
Length:648
Mass (Da):74,266
Last modified:July 5, 2004 - v1
Checksum:iE181EE42A0D39129
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017262 Genomic DNA. Translation: AAT03017.1.
RefSeqiYP_012840.1. NC_002973.6.

Genome annotation databases

EnsemblBacteriaiAAT03017; AAT03017; LMOf2365_0230.
GeneIDi2797333.
KEGGilmf:LMOf2365_0230.
PATRICi20321639. VBILisMon105049_0233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017262 Genomic DNA. Translation: AAT03017.1 .
RefSeqi YP_012840.1. NC_002973.6.

3D structure databases

ProteinModelPortali Q724J4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 265669.LMOf2365_0230.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT03017 ; AAT03017 ; LMOf2365_0230 .
GeneIDi 2797333.
KEGGi lmf:LMOf2365_0230.
PATRICi 20321639. VBILisMon105049_0233.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000217053.
KOi K15780.
OMAi NDISYPL.
OrthoDBi EOG693GNP.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_01161. tRNA_Ile_lys_synt.
InterProi IPR005904. Hxn_phspho_trans.
IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view ]
Pfami PF01171. ATP_bind_3. 1 hit.
PF00156. Pribosyltran. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view ]
SMARTi SM00977. TilS_C. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
TIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F2365.

Entry informationi

Entry nameiTILS_LISMF
AccessioniPrimary (citable) accession number: Q724J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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