Q724H7 (ISPD1_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1
MEP cytidylyltransferase 1
Short name=MCT 1

Gene names

Name:	ispD1
Synonyms:	ispD
Ordered Locus Names:	LMOf2365_0247

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 HAMAP MF_00108		PRO_0000075585
Sites
	Site	15	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	153	1	Positions MEP for the nucleophilic attack By similarity
	Site	209	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q724H7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B0A8E3D1681A0ED7	FASTA	232	25,898
	10 20 30 40 50 60 MNYELVFLAA GQGKRMNAQK NKMWLELVGE PIFIHALRPF LADNRCSKVI VVSQEEERKH 70 80 90 100 110 120 VKELMSQLNV AESRIEIVKG GSERQYSVAA GLERCGTERV VLVHDGARPF ITLDIIDRLL 130 140 150 160 170 180 IGVEQSKAAI CAVKVKDTVK RVVKGVVQET VDRENLWQVQ TPQAFELPIL RKAHQLARKE 190 200 210 220 230 QFLGTDEASL VERLPCPVAI VQGSYYNIKL TTPEDMPLAK AILGELGGIA ND

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT03034.1.
RefSeq	YP_012857.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q724H7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q724H7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2797930.
GenomeReviews	Gene locus LMOf2365_0247 in contig AE017262_GR.
KEGG	lmf:LMOf2365_0247.
PATRIC	20321707. VBILisMon105049_0252.
TIGR	LMOf2365_0247.
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	SKVIVVC.
PhylomeDB	Q724H7.
ProtClustDB	PRK13385.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_0247-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD1_LISMF

Accession

Primary (citable) accession number: Q724H7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program