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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110ZincUniRule annotation1
Metal bindingi112ZincUniRule annotation1
Metal bindingi137ZincUniRule annotation1
Metal bindingi139ZincUniRule annotation1
Binding sitei257ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:LMOf2365_0249
OrganismiListeria monocytogenes serotype 4b (strain F2365)
Taxonomic identifieri265669 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001195931 – 491Glutamate--tRNA ligaseAdd BLAST491

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ724H5
SMRiQ724H5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 23"HIGH" regionUniRule annotationAdd BLAST11
Motifi254 – 258"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252720
KOiK09698
OMAiAYYAFDT

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q724H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETKRVRVR YAPSPTGFLH IGNARTALFN YLFARHNDGD FIIRIEDTDA
60 70 80 90 100
KRNIADGEES QMTNLKWLGM DWDEGVDVPG KYGPYRQSER QSIYEPLIQQ
110 120 130 140 150
LLDEGLAYKC YCTEEELDAE REKQKANGEM PRYSGKCRHL TKEQQAEKEA
160 170 180 190 200
QGFKPSIRFK VPANETITFN DMVKDDVSFE SNGIGDFVIA KKDGIPTYNF
210 220 230 240 250
AVAVDDHLME ISHVLRGDDH ISNTPKQILI YNAFGWEPPI FGHMTLIVNE
260 270 280 290 300
SRRKLSKRDG SIIQFIEQYR DLGYLPEALF NFIAMLGWSP EGEEEIFSKE
310 320 330 340 350
EFIKMFDPKR LSKSPALFDN VKLTWVNNQY VKKLPLNDVV ELSLPHLQKA
360 370 380 390 400
GVVSAELDQA ELDWVHKLVS LYHEQMSYGA EIVPLSEMFF ADAESITFDE
410 420 430 440 450
EETAVLAEET VPTVISAFKK ELEALEVLEA AEVKAAIKRV QKETGVKGKG
460 470 480 490
LFMPIRIVTT GEMHGPELPL AIEVLGREKV LNRLDTWLKN N
Length:491
Mass (Da):55,979
Last modified:July 5, 2004 - v1
Checksum:i1EFE592C67FF60C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA Translation: AAT03036.1
RefSeqiWP_003728082.1, NC_002973.6

Genome annotation databases

EnsemblBacteriaiAAT03036; AAT03036; LMOf2365_0249
KEGGilmf:LMOf2365_0249

Similar proteinsi

Entry informationi

Entry nameiSYE_LISMF
AccessioniPrimary (citable) accession number: Q724H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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