Q72497 (Q72497_9HIV1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Gag polyprotein EMBL AAB60571.1

Gene names

Name:

gag EMBL AAB60571.1

Organism

Human immunodeficiency virus 1 EMBL AAB60571.1

Taxonomic identifier

11676 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Primate lentivirus group

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex By similarity. SAAS SAAS012344 Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers By similarity. SAAS SAAS012344
Subcellular location	Virion By similarity SAAS SAAS000721. Virion By similarity RuleBase RU004487. Virion By similarity SAAS SAAS000721.
Post-translational modification	Specific enzymatic cleavages by the viral protease yield mature proteins By similarity. RuleBase RU004487
Sequence similarities	Contains 2 CCHC-type zinc fingers. SAAS SAAS008916

Ontologies

Keywords
Cellular component	Host cytoplasm SAAS SAAS000721 RuleBase RU004487 Host nucleus SAAS SAAS000721 RuleBase RU004487 Virion
Coding sequence diversity	Ribosomal frameshifting SAAS SAAS000721
Disease	AIDS RuleBase RU004310
Domain	Repeat SAAS SAAS008916 Zinc-finger RuleBase RU000338 SAAS SAAS010999
Ligand	Metal-binding RNA-binding SAAS SAAS000721 Viral nucleoprotein RuleBase RU004487 Zinc
Molecular function	Capsid protein RuleBase RU000316 SAAS SAAS000721
Technical term	3D-structure PDB 1L6N PDB 1M9X PDB 2JMG PDB 2NV3 PDB 1M9E PDB 1M9F PDB 1M9Y PDB 1M9C PDB 1M9D PDB 2BUO PDB 3OBX PDB 3OBU
Gene Ontology (GO)
Biological process	viral reproduction Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell nucleus Inferred from electronic annotation. Source: UniProtKB-KW viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.5. Source: IntAct structural molecule activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPIA	P62937	6	EBI-1036263,EBI-437708	From a different organism.

Sequences

	Sequence		Length	Mass (Da)
	Q72497 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 08F450AC834088C6	FASTA	500	55,847
	10 20 30 40 50 60 MGARASVLSG GELDKWEKIR LRPGGKKQYK LKHIVWASRE LERFAVNPGL LETSEGCRQI 70 80 90 100 110 120 LGQLQPSLQT GSEELRSLYN TIAVLYCVHQ RIDVKDTKEA LDKIEEEQNK SKKKAQQAAA 130 140 150 160 170 180 DTGNNSQVSQ NYPIVQNLQG QMVHQAISPR TLNAWVKVVE EKAFSPEVIP MFSALSEGAT 190 200 210 220 230 240 PQDLNTMLNT VGGHQAAMQM LKETINEEAA EWDRLHPVHA GPIAPGQMRE PRGSDIAGTT 250 260 270 280 290 300 STLQEQIGWM THNPPIPVGE IYKRWIILGL NKIVRMYSPT SILDIRQGPK EPFRDYVDRF 310 320 330 340 350 360 YKTLRAEQAS QEVKNWMTET LLVQNANPDC KTILKALGPG ATLEEMMTAC QGVGGPGHKA 370 380 390 400 410 420 RVLAEAMSQV TNPATIMIQK GNFRNQRKTV KCFNCGKEGH IAKNCRAPRK KGCWKCGKEG 430 440 450 460 470 480 HQMKDCTERQ ANFLGKIWPS HKGRPGNFLQ SRPEPTAPPE ESFRFGEETT TPSQRQEPID 490 500 KELYPLASLR SLFGSDPSSQ

References

[1]	"Recovery of virtually full-length HIV-1 provirus of diverse subtypes from primary virus cultures using the polymerase chain reaction." Salminen M.O., Koch C., Sanders-Buell E., Ehrenberg P.K., Michael N.L., Carr J.K., Burke D.S., McCutchan F.E. Virology 213:80-86(1995) [PubMed: 7483282] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NL4-3 EMBL AAB60571.1.
[2]	"Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." Adachi A., Gendelman H.E., Koenig S., Folks T., Willey R., Rabson A., Martin M.A. J. Virol. 59:284-291(1986) [PubMed: 3016298] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: NL4-3 EMBL AAB60571.1.
[3]	Salminen M.S. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: NL4-3 EMBL AAB60571.1.
[4]	"Structure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein." Tang C., Ndassa Y., Summers M.F. Nat. Struct. Biol. 9:537-543(2002) [PubMed: 12032547] [Abstract] Cited for: STRUCTURE BY NMR OF 1-283.
[5]	"Structural insights into the catalytic mechanism of cyclophilin A." Howard B.R., Vajdos F.F., Li S., Sundquist W.I., Hill C.P. Nat. Struct. Biol. 10:475-481(2003) [PubMed: 12730686] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 133-278.
[6]	"Point mutations in the HIV-1 matrix protein turn off the myristyl switch." Saad J.S., Loeliger E., Luncsford P., Liriano M., Tai J., Kim A., Miller J., Joshi A., Freed E.O., Summers M.F. J. Mol. Biol. 366:574-585(2007) [PubMed: 17188710] [Abstract] Cited for: STRUCTURE BY NMR OF 2-132.
[7]	"The HIV-1 capsid protein C-terminal domain in complex with a virus assembly inhibitor." Ternois F., Sticht J., Duquerroy S., Krausslich H.G., Rey F.A. Nat. Struct. Mol. Biol. 12:678-682(2005) [PubMed: 16041386] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 278-363.
[8]	"Crystallographic and functional analysis of the ESCRT-I /HIV-1 Gag PTAP interaction." Im Y.J., Kuo L., Ren X., Burgos P.V., Zhao X.Z., Liu F., Burke T.R., Bonifacino J.S., Freed E.O., Hurley J.H. Structure 18:1536-1547(2010) [PubMed: 21070952] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 453-461.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U26942 Genomic DNA. Translation: AAB60571.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1L6N	NMR	-	A	1-283	[»]
1M9C	X-ray	2.00	C/D	133-278	[»]
1M9D	X-ray	1.90	C/D	133-278	[»]
1M9E	X-ray	1.72	C/D	133-278	[»]
1M9F	X-ray	1.73	C/D	133-278	[»]
1M9X	X-ray	1.70	C/D/G/H	133-278	[»]
1M9Y	X-ray	1.90	C/D/G/H	133-278	[»]
2BUO	X-ray	1.70	A	278-363	[»]
2JMG	NMR	-	A	2-132	[»]
2NV3	NMR	-	A	2-132	[»]
3OBU	X-ray	1.60	B	453-461	[»]
3OBX	X-ray	1.60	B	453-461	[»]

ProteinModelPortal

Q72497.

SMR

Q72497. Positions 2-432, 449-500.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-36777N.

IntAct

Q72497. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000721. Gag_p24.
IPR014817. Gag_p6.
IPR000071. Lentvrl_matrix_N.
IPR012344. Matrix_N_HIV/RSV.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR013084. Znf_CCH_retrovir.
IPR001878. Znf_CCHC.
[Graphical view]

Gene3D

G3DSA:1.10.150.90. Matrix_HIV/RSV_N. 1 hit.
G3DSA:1.10.1200.30. Retrov_capsid_C. 1 hit.
G3DSA:1.10.375.10. Retrov_capsid_N. 1 hit.
G3DSA:4.10.60.10. Znf_CCH_retrovir. 1 hit.

Pfam

PF00540. Gag_p17. 1 hit.
PF00607. Gag_p24. 1 hit.
PF08705. Gag_p6. 1 hit.
PF00098. zf-CCHC. 2 hits.
[Graphical view]

PRINTS

PR00234. HIV1MATRIX.

SMART

SM00343. ZnF_C2HC. 2 hits.
[Graphical view]

SUPFAM

SSF47353. Retrov_capsid_C. 1 hit.
SSF47943. Retrov_capsid_N. 1 hit.
SSF47836. Retrovir_matrix. 1 hit.

PROSITE

PS50158. ZF_CCHC. 2 hits.
[Graphical view]

ProtoNet

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Entry information

Entry name

Q72497_9HIV1

Accession

Primary (citable) accession number: Q72497

Entry history

Integrated into UniProtKB/TrEMBL:	November 1, 1996
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)