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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42Substrate; via amide nitrogenUniRule annotation1
Binding sitei118ATPUniRule annotation1
Binding sitei164ATPUniRule annotation1
Binding sitei191Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLMON265669:G1G0V-350-MONOMER
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:LMOf2365_0334
OrganismiListeria monocytogenes serotype 4b (strain F2365)
Taxonomic identifieri265669 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569441 – 269Hydroxyethylthiazole kinaseAdd BLAST269

Proteomic databases

PRIDEiQ723Z1

Structurei

3D structure databases

ProteinModelPortaliQ723Z1
SMRiQ723Z1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000114352
KOiK00878

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

Q723Z1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDFMTLEKV WEKGPLVHNI TNIVVANDSA NGLLAIGASP IMGSAKEEMD
60 70 80 90 100
ELAKMADVLV INIGTLDGEL VTAMKIAGRA ANVAGTPVVL DPVGVGATSY
110 120 130 140 150
RRKVVQELLA EIQFTAIRGN AGELAAIAGE AWEAKGVDAG VGSADVLSIA
160 170 180 190 200
EKVANEWSTV VIISGEVDVI SDGTRFAKVA NGSALLPRIT GSGCLLSAVC
210 220 230 240 250
GSFMAVQDDA FRASVEACAS YAVASEYAEI ELERKLPGSF RPQFLDALAS
260
WSVEKTRAKA KIQESGEHK
Length:269
Mass (Da):28,185
Last modified:June 7, 2005 - v2
Checksum:iC5A9CBA1950A27DD
GO

Sequence cautioni

The sequence AAT03120 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA Translation: AAT03120.1 Different initiation.
RefSeqiWP_048813147.1, NC_002973.6

Genome annotation databases

EnsemblBacteriaiAAT03120; AAT03120; LMOf2365_0334
KEGGilmf:LMOf2365_0334

Similar proteinsi

Entry informationi

Entry nameiTHIM_LISMF
AccessioniPrimary (citable) accession number: Q723Z1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: May 23, 2018
This is version 84 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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