Q723E1 (KPRS2_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 2
Short name=RPPK 2
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 2
Short name=P-Rib-PP synthase 2
Short name=PRPP synthase 2

Gene names

Name:	prs2
Ordered Locus Names:	LMOf2365_0538

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Ribose-phosphate pyrophosphokinase 2 HAMAP MF_00583_B		PRO_0000141156
Regions
	Region	212 – 225	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	129	1	Magnesium Potential
	Metal binding	131	1	Magnesium Potential
	Metal binding	140	1	Magnesium Potential
	Metal binding	144	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q723E1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 82F9270DB1B0D529	FASTA	311	34,346
	10 20 30 40 50 60 MAGKMKLFSV TSERPLATKI ADYLDIPLCE VELQKFSDGE VKINIEESIR GTNAYVVQSM 70 80 90 100 110 120 NSNVNERLME LLIMVDALKR ASVHSINIIM PYYGYARQDR KARSREPITA KLMANLIQRA 130 140 150 160 170 180 GADRLITVDL HAAQIQGFFN IPIDHLSAIP LIGDYLIENY GEKDVVVVAP DHSGVVRARR 190 200 210 220 230 240 IADRLNAPIA ILNRKPRPHE DEIMSVIGDV KGKVAIVVDD IIDTGVRATT SADILLEKGA 250 260 270 280 290 300 VEVIACATHS VMAGNATERL QNSNIKEVIT SDSIDLPEDK QFDKLTTISI GRILGRAIEG 310 VQENRSLHPL F

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT03320.1.
RefSeq	YP_013143.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q723E1.
SMR	Q723E1. Positions 4-311.
ModBase	Search...
Protein-protein interaction databases
STRING	Q723E1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2798065.
GenomeReviews	Gene locus LMOf2365_0538 in contig AE017262_GR.
KEGG	lmf:LMOf2365_0538.
PATRIC	20322281. VBILisMon105049_0538.
TIGR	LMOf2365_0538.
Phylogenomic databases
eggNOG	COG0462.
HOGENOM	HBG519284.
OMA	ELMIMIN.
PhylomeDB	Q723E1.
ProtClustDB	CLSK563942.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_0538-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_LISMF

Accession

Primary (citable) accession number: Q723E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program