Q722H1 (GLMS_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
EC=2.6.1.16

Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase

Gene names

Name:	glmS
Ordered Locus Names:	LMOf2365_0762

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity. HAMAP-Rule MF_00164
Catalytic activity	L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate. HAMAP-Rule MF_00164
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00164
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00164.
Sequence similarities	Contains 1 glutamine amidotransferase type-2 domain. Contains 2 SIS domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase Repeat
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro glutamine-fructose-6-phosphate transaminase (isomerizing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 601	600	Glutamine--fructose-6-phosphate aminotransferase [isomerizing] HAMAP-Rule MF_00164		PRO_0000135352
Regions
	Domain	2 – 216	215	Glutamine amidotransferase type-2
	Domain	282 – 421	140	SIS 1
	Domain	453 – 591	139	SIS 2
Sites
	Active site	2	1	Nucleophile; for GATase activity By similarity
	Active site	596	1	For Fru-6P isomerization activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q722H1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: AD427F6F6812EA15	FASTA	601	65,647
	10 20 30 40 50 60 MCGIVGYIGT NNAKSILLEG LEKLEYRGYD SAGIALQNKD LVTVVKEKGR IADLASLVPS 70 80 90 100 110 120 DAFGTTGIGH TRWATHGKPN HENAHPHQSK SGRFTMVHNG VIENYTLLKE EYLKNHSFVS 130 140 150 160 170 180 DTDTEVIVQL IELFAAELST KEAFKKALSL LHGSYAICLI DQTDTETLYA AKNKSPLLIG 190 200 210 220 230 240 KGENFNVIAS DAMAVLKETD EFVEIMDKEI VIVTKDGFTL ETLEGEEISR ASYTAELDAS 250 260 270 280 290 300 DIEKGTYPHY MLKEIDEQPA VTRKIIQAYQ NEAGEINVDQ TILDEILSSD RIHIVACGTS 310 320 330 340 350 360 YHAGLVGKNL IEKMAKIPVE VHVSSEFGYN LPLMSKKPLF IFITQSGETA DSRQCLVKVK 370 380 390 400 410 420 ELGYRTLTLT NVPGSTLDRE ADHSMYLYAG PEIAVASTKA YTAQISVLAV LAVSLGREIG 430 440 450 460 470 480 DAEALSINLA AELGIVATAM EAMVSSKEVI EHIAGEYLAT SRNAFFLGRN IDYFVAMEAA 490 500 510 520 530 540 LKLKEISYIQ AEGFASGELK HGTIALIEDG TPVLALITQE SINWNIRGNV NEVLARGAKT 550 560 570 580 590 600 CVFAMENVAQ PGDRFVIPQV HPLLTPLASV IPCQLLAYYA ALHRDCDVDK PRNLAKSVTV E

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT03543.1.
RefSeq	YP_013366.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q722H1.
ModBase	Search...
Protein-protein interaction databases
STRING	265669.LMOf2365_0762.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT03543; AAT03543; LMOf2365_0762.
GeneID	2798214.
KEGG	lmf:LMOf2365_0762.
PATRIC	20322738. VBILisMon105049_0764.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0449.
HOGENOM	HOG000258896.
KO	K00820.
OMA	CLQGLER.
ProtClustDB	PRK00331.
Family and domain databases
HAMAP	MF_00164. GlmS.
InterPro	IPR017932. GATase_2_dom. IPR005855. GlmS_trans. IPR001347. SIS. [Graphical view]
PANTHER	PTHR10937:SF0. PTHR10937:SF0. 1 hit.
Pfam	PF01380. SIS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01135. glmS. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS51464. SIS. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLMS_LISMF

Accession

Primary (citable) accession number: Q722H1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program