Q721S9 (ALR_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	LMOf2365_0905

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Alanine racemase HAMAP MF_01201		PRO_0000114533
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	263	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q721S9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: FA19DE48C2D9F93B	FASTA	368	41,068
	10 20 30 40 50 60 MVTGWHRPTW IEIDRAAIRE NIKNEQNKLP ENVDLWAVVK ANAYGHGIIE VARTAKEAGA 70 80 90 100 110 120 KGFCVAILDE ALALREAGFQ DDFILVLGAT RKEDANLAAK NHISLTVFRE DWLEELTLEA 130 140 150 160 170 180 PLRIHLKVDS GMGRLGIRTT DEARRIETTI AKDNQLQLEG IYTHFATADQ LETSYFEQQL 190 200 210 220 230 240 AKFQTILTSL KNRPTYVHTA NSAASLLQPQ IGFDAIRFGI SMYGLTPSTE IKTSLPFELK 250 260 270 280 290 300 PALALYTEMV HVKELAPGDS VSYGATYTAT EREWVATLPI GYADGLIRHY SGFHVLVDGE 310 320 330 340 350 360 LAPIIGRVCM DQTIIKLPRE FQTGSKVTII GTDHGNTITA DDAAHYLDTI NYEVTCLLNE RIPRKYIH

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT03685.1.
RefSeq	YP_013508.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q721S9.
SMR	Q721S9. Positions 4-367.
ModBase	Search...
Protein-protein interaction databases
STRING	Q721S9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2798069.
GenomeReviews	Gene locus LMOf2365_0905 in contig AE017262_GR.
KEGG	lmf:LMOf2365_0905.
PATRIC	20323026. VBILisMon105049_0908.
TIGR	LMOf2365_0905.
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	TRKEDAN.
PhylomeDB	Q721S9.
ProtClustDB	CLSK564147.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_0905-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LISMF

Accession

Primary (citable) accession number: Q721S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program