Q720Y7 (ISPD2_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2
MEP cytidylyltransferase 2
Short name=MCT 2

Gene names

Name:	ispD2
Ordered Locus Names:	LMOf2365_1100

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 237

237

Putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 HAMAP MF_00108

PRO_0000075586

Sites

Site

14

1

Transition state stabilizer By similarity

Site

22

1

Transition state stabilizer By similarity

Site

159

1

Positions MEP for the nucleophilic attack By similarity

Site

216

1

Positions MEP for the nucleophilic attack By similarity

Secondary structure

1

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237

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

22 – 24

3

Helix

32 – 41

10

Beta strand

47 – 52

6

Helix

55 – 57

3

Helix

58 – 68

11

Beta strand

74 – 77

4

Helix

83 – 88

6

Helix

92 – 97

6

Beta strand

105 – 110

6

Helix

118 – 130

13

Beta strand

134 – 136

3

Beta strand

148 – 152

5

Helix

159 – 161

3

Beta strand

162 – 164

3

Beta strand

169 – 172

4

Helix

173 – 181

9

Helix

185 – 190

6

Helix

194 – 200

7

Beta strand

206 – 208

3

Helix

220 – 233

14

Sequences

	Sequence		Length	Mass (Da)
	Q720Y7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 72A1EEA5300BE053	FASTA	237	26,793
	10 20 30 40 50 60 MIYAQILAGG KGTRMGNVSM PKQFLPLNGK PIIVHTVEKF ILNTRFDKIL ISSPKEWMNH 70 80 90 100 110 120 AEDNIKKYIS DDRIVVIEGG EDRNETIMNG IRFVEKTYGL TDDDIIVTHD AVRPFLTHRI 130 140 150 160 170 180 IEENIDAALE TGAVDTVIEA LDTIVESSNH EVITDIPVRD HMYQGQTPQS FNMKKVFNHY 190 200 210 220 230 QNLTPEKKQI LTDACKICLL AGDDVKLVKG EIFNIKITTP YDLKVANAII QERIAND

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE017262 Genomic DNA. Translation: AAT03877.1.

RefSeq

YP_013700.1. NC_002973.6.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3F1C	X-ray	2.30	A/B	2-236	[»]

ProteinModelPortal

Q720Y7.

ModBase

Search...

Protein-protein interaction databases

STRING

Q720Y7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2799328.

GenomeReviews

Gene locus LMOf2365_1100 in contig AE017262_GR.

KEGG

lmf:LMOf2365_1100.

PATRIC

20323436. VBILisMon105049_1107.

TIGR

LMOf2365_1100.

Phylogenomic databases

eggNOG

COG1211.

HOGENOM

HBG672839.

OMA

GELFNIK.

PhylomeDB

Q720Y7.

ProtClustDB

PRK00155.

Enzyme and pathway databases

BioCyc

LMON265669:LMOF2365_1100-MONOMER.

Family and domain databases

HAMAP

MF_00108. IspD.
[Tree]

InterPro

IPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
[Graphical view]

KO

K00991.

Pfam

PF01128. IspD. 1 hit.
[Graphical view]

PROSITE

PS01295. ISPD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ISPD2_LISMF

Accession

Primary (citable) accession number: Q720Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program