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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:LMOf2365_1294
OrganismiListeria monocytogenes serotype 4b (strain F2365)
Taxonomic identifieri265669 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117252Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ720E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q720E5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKSVNVIGA GLAGSEAAWQ LVKRGVKVDL YEMRPVKQTP AHHTDKFAEL
60 70 80 90 100
VCTNSLRANG LTNAVGVIKE EMRILDSIII EAADKASVPA GGALAVDRHE
110 120 130 140 150
FSGYITDKVK NHPLVTVHTE EVTTIPEGPT IIATGPLTSP ALADEIKQLT
160 170 180 190 200
GEEYLYFYDA AAPIIEKDSI DMDKVYLKSR YDKGEAAYLN CPMSEEEFNA
210 220 230 240 250
FYEALVTAET AALKEFEKEV FFEGCMPIEV MAKRGIKTML FGPLKPVGLE
260 270 280 290 300
DPKTGKRPYA VLQLRQDDAA GTLYNMVGFQ THLKWGEQKR VFGMIPGLEN
310 320 330 340 350
AEIVRYGVMH RNTFINSPTV LEPTYQLKTR NDLFFAGQMT GVEGYVESAA
360 370 380 390 400
SGLAAGINAA NFVEEKELVV FPAETAIGSL AHYITSASKK SFQPMNVNFG
410 420 430
LFPELETKIR AKQERNEKLA ERALNAIKRV AEEL
Length:434
Mass (Da):47,852
Last modified:July 5, 2004 - v1
Checksum:iDAB5B0400DF43EAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA. Translation: AAT04069.1.

Genome annotation databases

EnsemblBacteriaiAAT04069; AAT04069; LMOf2365_1294.
PATRICi20323821. VBILisMon105049_1298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017262 Genomic DNA. Translation: AAT04069.1.

3D structure databases

ProteinModelPortaliQ720E5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT04069; AAT04069; LMOf2365_1294.
PATRICi20323821. VBILisMon105049_1298.

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: F2365.

Entry informationi

Entry nameiTRMFO_LISMF
AccessioniPrimary (citable) accession number: Q720E5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.