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Q71ZR3

- PFLA_LISMF

UniProt

Q71ZR3 - PFLA_LISMF

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Protein
Pyruvate formate-lyase-activating enzyme
Gene
pflA, LMOf2365_1426
Organism
Listeria monocytogenes serotype 4b (strain F2365)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi35 – 351Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi38 – 381Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
    Short name:
    PFL-activating enzyme
    Gene namesi
    Name:pflA
    Ordered Locus Names:LMOf2365_1426
    OrganismiListeria monocytogenes serotype 4b (strain F2365)
    Taxonomic identifieri265669 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000000563: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 248248Pyruvate formate-lyase-activating enzyme
    PRO_0000200531Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi265669.LMOf2365_1426.

    Structurei

    3D structure databases

    ProteinModelPortaliQ71ZR3.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1180.
    HOGENOMiHOG000011458.
    KOiK04069.
    OMAiWEMETNK.
    OrthoDBiEOG64FKHC.

    Family and domain databases

    InterProiIPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02493. PFLA. 1 hit.
    PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q71ZR3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEVLGRVHS VETMGTVDGP GIRFIVFMQG CLLRCQFCHN PDTWKIGTGT    50
    ERSAQDVFDE AIKYKEFWDA SGGGVTVSGG EPLLQVDFLI EFFTLCKAAG 100
    VHTTIDSCGG CFTRDPEFIE KLDRLMEVTD LILLDIKQIN PEKHLKLTTK 150
    SNAPIIDFAH YLRDKEQPIW IRHVLIPTKT DDPEDLTKLH EFIQTLPNVK 200
    QVDVLPYHTM GVYKWKEMGI RYPLEGIEAP EEEVVALANK ILETSSYK 248
    Length:248
    Mass (Da):28,115
    Last modified:July 5, 2004 - v1
    Checksum:i0A6F5BBA95CBEBC9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017262 Genomic DNA. Translation: AAT04201.1.
    RefSeqiYP_014024.1. NC_002973.6.

    Genome annotation databases

    EnsemblBacteriaiAAT04201; AAT04201; LMOf2365_1426.
    GeneIDi2798698.
    KEGGilmf:LMOf2365_1426.
    PATRICi20324091. VBILisMon105049_1433.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017262 Genomic DNA. Translation: AAT04201.1 .
    RefSeqi YP_014024.1. NC_002973.6.

    3D structure databases

    ProteinModelPortali Q71ZR3.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 265669.LMOf2365_1426.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT04201 ; AAT04201 ; LMOf2365_1426 .
    GeneIDi 2798698.
    KEGGi lmf:LMOf2365_1426.
    PATRICi 20324091. VBILisMon105049_1433.

    Phylogenomic databases

    eggNOGi COG1180.
    HOGENOMi HOG000011458.
    KOi K04069.
    OMAi WEMETNK.
    OrthoDBi EOG64FKHC.

    Family and domain databases

    InterProi IPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02493. PFLA. 1 hit.
    PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: F2365.

    Entry informationi

    Entry nameiPFLA_LISMF
    AccessioniPrimary (citable) accession number: Q71ZR3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: July 5, 2004
    Last modified: May 14, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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