Q71ZN5 (DAPA_LISMF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	LMOf2365_1454

Organism

Listeria monocytogenes serotype 4b (strain F2365) [Complete proteome] [HAMAP]

Taxonomic identifier

265669 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000103124
Regions
	Region	50 – 51	2	Pyruvate binding By similarity
Sites
	Active site	164	1	Schiff-base intermediate with substrate By similarity
	Binding site	109	1	Pyruvate By similarity
	Site	136	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q71ZN5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 78485DDA56B51394	FASTA	293	31,420
	10 20 30 40 50 60 MDLGKVITAM VTPIHPEKDK VCKKRIHHLV NHLIKNGSDG LVIAGTTGES PTLSHDEKIK 70 80 90 100 110 120 LFRQVIETND GRAKLIAGTG SNNTAETIAF TKEVAELGGI DAVLIVAPYY NKPNQDGLYA 130 140 150 160 170 180 HFAAVSEASD LPVVIYNIPG RSVVNIEPET IIRLAKLPNI VGVKESSGNL DNISKIIAET 190 200 210 220 230 240 SDDFQVYSGD DSLTLPILAV GGNGVISVAS HVVGNEMQEM IQAFERGEVQ KAAQIHRELL 250 260 270 280 290 PLMNGLFSVP NPAPTKYLLN QQGISVGPVR LPLVDLNAEQ GTKLQAILEG LSK

References

[1]

"Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species." Nelson K.E., Fouts D.E., Mongodin E.F., Ravel J., DeBoy R.T., Kolonay J.F., Rasko D.A., Angiuoli S.V., Gill S.R., Paulsen I.T., Peterson J.D., White O., Nelson W.C., Nierman W.C., Beanan M.J., Brinkac L.M., Daugherty S.C., Dodson R.J. , Durkin A.S., Madupu R., Haft D.H., Selengut J., Van Aken S.E., Khouri H.M., Fedorova N., Forberger H.A., Tran B., Kathariou S., Wonderling L.D., Uhlich G.A., Bayles D.O., Luchansky J.B., Fraser C.M. Nucleic Acids Res. 32:2386-2395(2004) [PubMed: 15115801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F2365.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017262 Genomic DNA. Translation: AAT04229.1.
RefSeq	YP_014052.1. NC_002973.6.
3D structure databases
ProteinModelPortal	Q71ZN5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q71ZN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2799279.
GenomeReviews	Gene locus LMOf2365_1454 in contig AE017262_GR.
KEGG	lmf:LMOf2365_1454.
PATRIC	20324147. VBILisMon105049_1461.
TIGR	LMOf2365_1454.
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	CEMEDSN.
PhylomeDB	Q71ZN5.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	LMON265669:LMOF2365_1454-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_LISMF

Accession

Primary (citable) accession number: Q71ZN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program